A highly specific tool for identification of Xanthomonas vasicola pv. musacearum based on five Xvm-specific coding sequences
dc.contributor.author | Nakato, GV | |
dc.contributor.author | Wicker, E | |
dc.contributor.author | Coutinho, TA | |
dc.contributor.author | Mahuku, G | |
dc.contributor.author | Studholme, DJ | |
dc.date.accessioned | 2019-02-04T10:21:25Z | |
dc.date.issued | 2018-12-27 | |
dc.description.abstract | Xanthomonas vasicola pv. musacearum (Xvm) is a bacterial pathogen responsible for the economically important Xanthomonas wilt disease on banana and enset crops in Sub-Saharan Africa. Given that the symptoms are similar to those of other diseases, molecular diagnosis is essential to unambiguously identify this pathogen and distinguish it from closely related strains not pathogenic on these hosts. Currently, Xvm identification is based on polymerase chain reaction (PCR) with GspDm primers, targeting the gene encoding general secretory protein D. Experimental results and examination of genomic sequences revealed poor specificity of the GspDm PCR. Here, we present and validate five new Xvm-specific primers amplifying only Xvm strains. | en_GB |
dc.description.sponsorship | Agropolis Fondation | en_GB |
dc.identifier.citation | Vol. 4 (12), article e01080 | en_GB |
dc.identifier.doi | 10.1016/j.heliyon.2018.e01080 | |
dc.identifier.grantnumber | 1605-025 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/35705 | |
dc.language.iso | en | en_GB |
dc.publisher | Elsevier | en_GB |
dc.rights | © 2018 Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | en_GB |
dc.subject | Bioinformatics | en_GB |
dc.subject | Microbiology | en_GB |
dc.subject | Molecular biology | en_GB |
dc.subject | Plant biology | en_GB |
dc.title | A highly specific tool for identification of Xanthomonas vasicola pv. musacearum based on five Xvm-specific coding sequences | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2019-02-04T10:21:25Z | |
dc.identifier.issn | 2405-8440 | |
dc.description | This is the final version. Available on open access from Elsevier via the DOI in this record | en_GB |
dc.identifier.journal | Heliyon | en_GB |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en_GB |
dcterms.dateAccepted | 2018-12-18 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2018-12-18 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2019-02-04T10:17:39Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2019-02-04T10:21:27Z | |
refterms.panel | A | en_GB |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's licence is described as © 2018 Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license
(http://creativecommons.org/licenses/by-nc-nd/4.0/).