Evolutionary diversification of the RomR protein of the invasive deltaproteobacterium, Bdellovibrio bacteriovorus
dc.contributor.author | Lowry, RC | |
dc.contributor.author | Milner, DS | |
dc.contributor.author | Al-Bayati, A | |
dc.contributor.author | Lambert, C | |
dc.contributor.author | Francis, VI | |
dc.contributor.author | Porter, SL | |
dc.contributor.author | Sockett, RE | |
dc.date.accessioned | 2019-03-01T15:58:48Z | |
dc.date.issued | 2019-03-21 | |
dc.description.abstract | Bdellovibrio bacteriovorus is a predatory deltaproteobacterium that encounters individual Gram-negative prey bacteria with gliding or swimming motility, and then is able to invade such prey cells via type IVa pilus-dependent mechanisms. Movement control (pili or gliding) in other deltaproteobacteria, such as the pack hunting Myxococcus xanthus, uses a response regulator protein, RomRMx (which dynamically relocalises between the cell poles) and a GTPase, MglAMx, previously postulated as an interface between the FrzMx chemosensory system and gliding or pilus-motility apparatus, to produce regulated bidirectional motility. In contrast, B. bacteriovorus predation is a more singular encounter between a lone predator and prey; contact is always via the piliated, non-flagellar pole of the predator, involving MglABd, but no Frz system. In this new study, tracking fluorescent RomRBd microscopically during predatory growth shows that it does not dynamically relocalise, in contrast to the M. xanthus protein; instead having possible roles in growth events. Furthermore, transcriptional start analysis, site-directed mutagenesis and bacterial two-hybrid interaction studies, indicate an evolutionary loss of RomRBd activation (via receiver domain phosphorylation) in this lone hunting bacterium, demonstrating divergence from its bipolar role in motility in pack-hunting M. xanthus and further evolution that may differentiate lone from pack predators. | en_GB |
dc.description.sponsorship | Leverhulme Trust | en_GB |
dc.description.sponsorship | Medical Research Council (MRC) | en_GB |
dc.description.sponsorship | Biotechnology and Biological Sciences Research Council (BBSRC) | en_GB |
dc.identifier.citation | Vol. 9, article 5007 | en_GB |
dc.identifier.doi | 10.1038/s41598-019-41263-5 | |
dc.identifier.grantnumber | RPG-2014-228 | en_GB |
dc.identifier.grantnumber | RPG-2014-241 | en_GB |
dc.identifier.grantnumber | BB/F016999/1 | en_GB |
dc.identifier.grantnumber | MR/M020045/1 | en_GB |
dc.identifier.grantnumber | BB/M010325/1 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/36178 | |
dc.language.iso | en | en_GB |
dc.publisher | Nature Research | en_GB |
dc.rights | © 2019 The Author(s). Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | |
dc.title | Evolutionary diversification of the RomR protein of the invasive deltaproteobacterium, Bdellovibrio bacteriovorus | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2019-03-01T15:58:48Z | |
dc.identifier.issn | 2045-2322 | |
dc.description | This is the final version. Available on open access from Nature Research via the DOI in this record | en_GB |
dc.identifier.journal | Scientific Reports | en_GB |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2019-02-27 | |
exeter.funder | ::Leverhulme Trust | en_GB |
exeter.funder | ::Medical Research Council (MRC) | en_GB |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2019-02-27 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2019-03-01T15:43:37Z | |
refterms.versionFCD | AM | |
refterms.dateFOA | 2019-03-21T14:00:37Z | |
refterms.panel | A | en_GB |
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Except where otherwise noted, this item's licence is described as © 2019 The Author(s). Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.