Genomic insights into neonicotinoid sensitivity in the solitary bee Osmia bicornis
dc.contributor.author | Beadle, K | |
dc.contributor.author | Singh, KS | |
dc.contributor.author | Troczka, BJ | |
dc.contributor.author | Randall, E | |
dc.contributor.author | Zaworra, M | |
dc.contributor.author | Zimmer, CT | |
dc.contributor.author | Hayward, A | |
dc.contributor.author | Reid, R | |
dc.contributor.author | Kor, L | |
dc.contributor.author | Kohler, M | |
dc.contributor.author | Buer, B | |
dc.contributor.author | Nelson, DR | |
dc.contributor.author | Williamson, MS | |
dc.contributor.author | Davies, TGE | |
dc.contributor.author | Field, LM | |
dc.contributor.author | Nauen, R | |
dc.contributor.author | Bass, C | |
dc.date.accessioned | 2019-03-06T15:45:28Z | |
dc.date.issued | 2019-02-04 | |
dc.description.abstract | The impact of pesticides on the health of bee pollinators is determined in part by the capacity of bee detoxification systems to convert these compounds to less toxic forms. For example, recent work has shown that cytochrome P450s of the CYP9Q subfamily are critically important in defining the sensitivity of honey bees and bumblebees to pesticides, including neonicotinoid insecticides. However, it is currently unclear if solitary bees have functional equivalents of these enzymes with potentially serious implications in relation to their capacity to metabolise certain insecticides. To address this question, we sequenced the genome of the red mason bee, Osmia bicornis, the most abundant and economically important solitary bee species in Central Europe. We show that O. bicornis lacks the CYP9Q subfamily of P450s but, despite this, exhibits low acute toxicity to the N-cyanoamidine neonicotinoid thiacloprid. Functional studies revealed that variation in the sensitivity of O. bicornis to N-cyanoamidine and N-nitroguanidine neonicotinoids does not reside in differences in their affinity for the nicotinic acetylcholine receptor or speed of cuticular penetration. Rather, a P450 within the CYP9BU subfamily, with recent shared ancestry to the Apidae CYP9Q subfamily, metabolises thiacloprid in vitro and confers tolerance in vivo. Our data reveal conserved detoxification pathways in model solitary and eusocial bees despite key differences in the evolution of specific pesticide-metabolising enzymes in the two species groups. The discovery that P450 enzymes of solitary bees can act as metabolic defence systems against certain pesticides can be leveraged to avoid negative pesticide impacts on these important pollinators. | en_GB |
dc.description.sponsorship | Biotechnology and Biological Science Research Council (BBSRC) | en_GB |
dc.description.sponsorship | Bayer AG | en_GB |
dc.description.sponsorship | European Research Council (ERC) | en_GB |
dc.identifier.citation | Vol. 15, e1007903 | en_GB |
dc.identifier.doi | 10.1371/journal.pgen.1007903 | |
dc.identifier.grantnumber | 15076182 | en_GB |
dc.identifier.grantnumber | BBS/OS/CP/000001 | en_GB |
dc.identifier.grantnumber | 646625 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/36329 | |
dc.language.iso | en | en_GB |
dc.publisher | Public Library of Science | en_GB |
dc.rights | Copyright: © 2019 Beadle et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en_GB |
dc.title | Genomic insights into neonicotinoid sensitivity in the solitary bee Osmia bicornis | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2019-03-06T15:45:28Z | |
dc.identifier.issn | 1553-7390 | |
dc.description | This is the final version. Available from the publisher via the DOI in this record. | en_GB |
dc.description | The Osmia bicornis whole genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession MPJT00000000. The RNAseq data generated in this study has been deposited in the Sequence Read Archive (SRA) under accession SRP065762. Accession numbers of the bee P450 genes manually curated in this study are shown in S5 Table. All other relevant data are within the paper and its Supporting Information files. | en_GB |
dc.identifier.journal | PLoS Genetics | en_GB |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2018-12-17 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2019-02-04 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2019-03-06T15:42:36Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2019-03-06T15:45:30Z | |
refterms.panel | A | en_GB |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's licence is described as Copyright: © 2019 Beadle et al. This is an open
access article distributed under the terms of the
Creative Commons Attribution License, which
permits unrestricted use, distribution, and
reproduction in any medium, provided the original
author and source are credited.