Addiction systems antagonize bacterial adaptive immunity.
dc.contributor.author | van Sluijs, L | |
dc.contributor.author | van Houte, S | |
dc.contributor.author | van der Oost, J | |
dc.contributor.author | Brouns, SJJ | |
dc.contributor.author | Buckling, A | |
dc.contributor.author | Westra, ER | |
dc.date.accessioned | 2019-04-08T10:46:31Z | |
dc.date.issued | 2019-03-05 | |
dc.description.abstract | CRISPR-Cas systems provide adaptive immunity against mobile genetic elements, but employment of this resistance mechanism is often reported with a fitness cost for the host. Whether or not CRISPR-Cas systems are important barriers for the horizontal spread of conjugative plasmids, which play a crucial role in the spread of antibiotic resistance, will depend on the fitness costs of employing CRISPR-based defences and the benefits of resisting conjugative plasmids. To estimate these costs and benefits we measured bacterial fitness associated with plasmid immunity using Escherichia coli and the conjugative plasmid pOX38-Cm. We find that CRISPR-mediated immunity fails to confer a fitness benefit in the absence of antibiotics, despite the large fitness cost associated with carrying the plasmid in this context. Similar to many other conjugative plasmids, pOX38-Cm carries a CcdAB toxin-antitoxin (TA) addiction system. These addiction systems encode long-lived toxins and short-lived anti-toxins, resulting in toxic effects following the loss of the TA genes from the bacterial host. Our data suggest that the lack of a fitness benefit associated with CRISPR-mediated defence is due to expression of the TA system before plasmid detection and degradation. As most antibiotic resistance plasmids encode TA systems this could have important consequences for the role of CRISPR-Cas systems in limiting the spread of antibiotic resistance. | en_GB |
dc.description.sponsorship | European Commission | en_GB |
dc.description.sponsorship | Biotechnology & Biological Sciences Research Council (BBSRC) | en_GB |
dc.description.sponsorship | Natural Environment Research Council | en_GB |
dc.description.sponsorship | Royal Society of Biological Sciences | en_GB |
dc.description.sponsorship | Netherlands Organization of Scientific Research (NWO) | en_GB |
dc.description.sponsorship | Wellcome Trust | en_GB |
dc.identifier.citation | Published online 5 March 2019 | en_GB |
dc.identifier.doi | 10.1093/femsle/fnz047 | |
dc.identifier.grantnumber | 752979 | en_GB |
dc.identifier.grantnumber | BB/R010781/1 | en_GB |
dc.identifier.grantnumber | NWO-TOP,854.10.003 | en_GB |
dc.identifier.grantnumber | NWO Vidi, 864.11.005 | en_GB |
dc.identifier.grantnumber | FP7/2007-2013 | en_GB |
dc.identifier.grantnumber | 327606 | en_GB |
dc.identifier.grantnumber | ERC-STG-2016-714478 - EVOIMMECH | en_GB |
dc.identifier.grantnumber | BB/N017412/1 | en_GB |
dc.identifier.grantnumber | 109776/Z/15/Z | en_GB |
dc.identifier.grantnumber | NE/M018350/1 | en_GB |
dc.identifier.other | 5369624 | |
dc.identifier.uri | http://hdl.handle.net/10871/36762 | |
dc.language.iso | en | en_GB |
dc.publisher | Oxford University Press (OUP) | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/30834930 | en_GB |
dc.rights | © FEMS 2019. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | en_GB |
dc.subject | CRISPR | en_GB |
dc.subject | TA | en_GB |
dc.subject | adaptive immunity | en_GB |
dc.subject | bacteria | en_GB |
dc.subject | plasmid | en_GB |
dc.subject | toxin | en_GB |
dc.title | Addiction systems antagonize bacterial adaptive immunity. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2019-04-08T10:46:31Z | |
exeter.place-of-publication | England | en_GB |
dc.description | This is the author accepted manuscript. The final version is available from Oxford University Press (OUP) via the DOI in this record. | en_GB |
dc.identifier.journal | FEMS Microbiology Letters | en_GB |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2019-03-05 | |
exeter.funder | ::European Commission | en_GB |
exeter.funder | ::Biotechnology & Biological Sciences Research Council (BBSRC) | en_GB |
rioxxterms.version | AM | en_GB |
rioxxterms.licenseref.startdate | 2019-03-05 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2019-04-08T10:38:16Z | |
refterms.versionFCD | AM | |
refterms.dateFOA | 2019-04-08T10:46:33Z | |
refterms.panel | A | en_GB |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's licence is described as © FEMS 2019. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.