Show simple item record

dc.contributor.authorBishop, A
dc.contributor.authorKamoshita, M
dc.contributor.authorPassmore, J
dc.contributor.authorHacker, C
dc.contributor.authorSchrader, T
dc.contributor.authorWaterham, H
dc.contributor.authorCostello, J
dc.contributor.authorSchrader, M
dc.date.accessioned2019-04-16T12:11:23Z
dc.date.issued2019-05-15
dc.description.abstractPeroxisomes and the endoplasmic reticulum (ER) cooperate extensively in lipid-related metabolic pathways, and the ER also provides phospholipids to enable the peroxisomal membrane to expand prior to division. Recently, we identified peroxisomal proteins ACBD5 and ACBD4, and the ER protein VAPB as tethering components which physically interact to foster peroxisome-ER associations at membrane contact sites. Overexpression or loss of these tether proteins alters the extent of peroxisome-ER interactions, impacting on lipid exchange between these two compartments. To facilitate further studies into peroxisome-ER associations at the level of membrane contact sites, their role, composition and regulation, we have developed two fluorescence-based systems to monitor peroxisome-ER interactions. We modified a proximity ligation assay and a split-fluorescence reporter system using split superfolder green fluorescent protein. Using the proximity ligation assay we were able to measure changes in peroxisome-ER interactions whilst the split-fluorescence reporter was more limited and only allowed us to label ER-peroxisome contacts. We show that both techniques can be useful additions to the toolkit of methods to study peroxisome-ER associations and explore the relative merits of each.en_GB
dc.description.sponsorshipBiotechnology & Biological Sciences Research Council (BBSRC)en_GB
dc.description.sponsorshipUniversity of Exeteren_GB
dc.description.sponsorshipJapan Society for the Promotion of Science (JSPS)en_GB
dc.description.sponsorshipZellweger UKen_GB
dc.description.sponsorshipSidney Perry Foundationen_GB
dc.description.sponsorshipDevon Educational Trusten_GB
dc.identifier.citationVol. 2. Published online 15 May 2019.en_GB
dc.identifier.doi10.1177/2515256419848641
dc.identifier.grantnumberBB/N01541X/1en_GB
dc.identifier.grantnumberH2020-MSCA-ITN-2018 812968 PERICOen_GB
dc.identifier.grantnumberSCP 3885en_GB
dc.identifier.urihttp://hdl.handle.net/10871/36828
dc.language.isoenen_GB
dc.publisherSAGE Publicationsen_GB
dc.rights© The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 License (http://www. creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
dc.subjectPeroxisomesen_GB
dc.subjectERen_GB
dc.subjectACBD5en_GB
dc.subjectVAPBen_GB
dc.subjectmembrane contact sitesen_GB
dc.titleFluorescent tools to analyse peroxisome-ER interactions in mammalian cellsen_GB
dc.typeArticleen_GB
dc.date.available2019-04-16T12:11:23Z
dc.descriptionThis is the final version. Available on open access from SAGE Publications via the DOI in this record.en_GB
dc.descriptionData availability: The research data supporting this publication are provided within this paper.en_GB
dc.identifier.eissn2515-2564
dc.identifier.journalContacten_GB
dc.rights.urihttp://www.creativecommons.org/licenses/by/4.0/en_GB
dcterms.dateAccepted2019-04-13
exeter.funder::Biotechnology & Biological Sciences Research Council (BBSRC)en_GB
rioxxterms.versionVoRen_GB
rioxxterms.licenseref.startdate2019-04-13
rioxxterms.typeJournal Article/Reviewen_GB
refterms.dateFCD2019-04-15T15:41:51Z
refterms.versionFCDAM
refterms.dateFOA2019-08-22T08:00:14Z
refterms.panelAen_GB


Files in this item

This item appears in the following Collection(s)

Show simple item record

© The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 License (http://www.
creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the
original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
Except where otherwise noted, this item's licence is described as © The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 License (http://www. creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).