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A design of experiments approach for the rapid formulation of a chemically defined medium for metabolic profiling of industrially important microbes

dc.contributor.authorSingleton, C
dc.contributor.authorGilman, J
dc.contributor.authorRollit, J
dc.contributor.authorZhang, K
dc.contributor.authorParker, DA
dc.contributor.authorLove, J
dc.date.accessioned2019-09-19T09:36:56Z
dc.date.issued2019-06-12
dc.description.abstractGeobacillus thermoglucosidans DSM2542 is an industrially important microbe, however the complex nutritional requirements of Geobacilli confound metabolic engineering efforts. Previous studies have utilised semi-defined media recipes that contain complex, undefined, biologically derived nutrients which have unknown ingredients that cannot be quantified during metabolic profiling. This study used design of experiments to investigate how individual nutrients and interactions between these nutrients contribute to growth. A mathematically derived defined medium has been formulated that has been shown to robustly support growth of G. thermoglucosidans in two different environmental conditions (96-well plate and shake flask) and with a variety of lignocellulose-based carbohydrates. This enabled the catabolism of industrially relevant carbohydrates to be investigated.en_GB
dc.description.sponsorshipShell Global Solutions BVen_GB
dc.description.sponsorshipUniversity of Exeteren_GB
dc.identifier.citationVol. 14 (6), article e0218208en_GB
dc.identifier.doi10.1371/journal.pone.0218208
dc.identifier.grantnumberJL-07721/14en_GB
dc.identifier.urihttp://hdl.handle.net/10871/38814
dc.language.isoenen_GB
dc.publisherPublic Library of Scienceen_GB
dc.rights© 2019 Singleton et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_GB
dc.titleA design of experiments approach for the rapid formulation of a chemically defined medium for metabolic profiling of industrially important microbesen_GB
dc.typeArticleen_GB
dc.date.available2019-09-19T09:36:56Z
dc.descriptionThis is the final version. Available on open access from Public Library of Science via the DOI in this recorden_GB
dc.descriptionData Availability: All relevant data are within the manuscript and its Supporting Information files.en_GB
dc.identifier.eissn1932-6203
dc.identifier.journalPLoS ONEen_GB
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_GB
dcterms.dateAccepted2019-05-28
rioxxterms.versionVoRen_GB
rioxxterms.licenseref.startdate2019-05-28
rioxxterms.typeJournal Article/Reviewen_GB
refterms.dateFCD2019-09-19T09:34:47Z
refterms.versionFCDVoR
refterms.dateFOA2019-09-19T09:37:06Z
refterms.panelAen_GB
refterms.depositExceptionpublishedGoldOA


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© 2019 Singleton et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's licence is described as © 2019 Singleton et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.