A design of experiments approach for the rapid formulation of a chemically defined medium for metabolic profiling of industrially important microbes
dc.contributor.author | Singleton, C | |
dc.contributor.author | Gilman, J | |
dc.contributor.author | Rollit, J | |
dc.contributor.author | Zhang, K | |
dc.contributor.author | Parker, DA | |
dc.contributor.author | Love, J | |
dc.date.accessioned | 2019-09-19T09:36:56Z | |
dc.date.issued | 2019-06-12 | |
dc.description.abstract | Geobacillus thermoglucosidans DSM2542 is an industrially important microbe, however the complex nutritional requirements of Geobacilli confound metabolic engineering efforts. Previous studies have utilised semi-defined media recipes that contain complex, undefined, biologically derived nutrients which have unknown ingredients that cannot be quantified during metabolic profiling. This study used design of experiments to investigate how individual nutrients and interactions between these nutrients contribute to growth. A mathematically derived defined medium has been formulated that has been shown to robustly support growth of G. thermoglucosidans in two different environmental conditions (96-well plate and shake flask) and with a variety of lignocellulose-based carbohydrates. This enabled the catabolism of industrially relevant carbohydrates to be investigated. | en_GB |
dc.description.sponsorship | Shell Global Solutions BV | en_GB |
dc.description.sponsorship | University of Exeter | en_GB |
dc.identifier.citation | Vol. 14 (6), article e0218208 | en_GB |
dc.identifier.doi | 10.1371/journal.pone.0218208 | |
dc.identifier.grantnumber | JL-07721/14 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/38814 | |
dc.language.iso | en | en_GB |
dc.publisher | Public Library of Science | en_GB |
dc.rights | © 2019 Singleton et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en_GB |
dc.title | A design of experiments approach for the rapid formulation of a chemically defined medium for metabolic profiling of industrially important microbes | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2019-09-19T09:36:56Z | |
dc.description | This is the final version. Available on open access from Public Library of Science via the DOI in this record | en_GB |
dc.description | Data Availability: All relevant data are within the manuscript and its Supporting Information files. | en_GB |
dc.identifier.eissn | 1932-6203 | |
dc.identifier.journal | PLoS ONE | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2019-05-28 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2019-05-28 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2019-09-19T09:34:47Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2019-09-19T09:37:06Z | |
refterms.panel | A | en_GB |
refterms.depositException | publishedGoldOA |
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Except where otherwise noted, this item's licence is described as © 2019 Singleton et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.