The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae
dc.contributor.author | Holt, CC | |
dc.contributor.author | Stone, M | |
dc.contributor.author | Bass, D | |
dc.contributor.author | Bateman, KS | |
dc.contributor.author | van Aerle, R | |
dc.contributor.author | Daniels, CL | |
dc.contributor.author | van der Giezen, M | |
dc.contributor.author | Ross, SH | |
dc.contributor.author | Hooper, C | |
dc.contributor.author | Stentiford, GD | |
dc.date.accessioned | 2019-10-04T15:22:37Z | |
dc.date.issued | 2019-07-12 | |
dc.description.abstract | Viral diseases of crustaceans are increasingly recognised as challenges to shellfish farms and fisheries. Here we describe the first naturally-occurring virus reported in any clawed lobster species. Hypertrophied nuclei with emarginated chromatin, characteristic histopathological lesions of DNA virus infection, were observed within the hepatopancreatic epithelial cells of juvenile European lobsters (Homarus gammarus). Transmission electron microscopy revealed infection with a bacilliform virus containing a rod shaped nucleocapsid enveloped in an elliptical membrane. Assembly of PCR-free shotgun metagenomic sequencing produced a circular genome of 107,063 bp containing 97 open reading frames, the majority of which share sequence similarity with a virus infecting the black tiger shrimp: Penaeus monodon nudivirus (PmNV). Multiple phylogenetic analyses confirm the new virus to be a novel member of the Nudiviridae: Homarus gammarus nudivirus (HgNV). Evidence of occlusion body formation, characteristic of PmNV and its closest relatives, was not observed, questioning the horizontal transmission strategy of HgNV outside of the host. We discuss the potential impacts of HgNV on juvenile lobster growth and mortality and present HgNV-specific primers to serve as a diagnostic tool for monitoring the virus in wild and farmed lobster stocks. | en_GB |
dc.description.sponsorship | Centre for Environment, Fisheries and Aquaculture Science (CEFAS) | en_GB |
dc.description.sponsorship | Innovate UK | en_GB |
dc.description.sponsorship | BBSRC | en_GB |
dc.identifier.citation | Vol. 9, 10086 | en_GB |
dc.identifier.doi | 10.1038/s41598-019-46008-y | |
dc.identifier.grantnumber | 102531 | en_GB |
dc.identifier.grantnumber | BB/N013891/1 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/39059 | |
dc.language.iso | en | en_GB |
dc.publisher | Nature Research (Springer Nature) | en_GB |
dc.rights | © The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre-ative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not per-mitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | en_GB |
dc.title | The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2019-10-04T15:22:37Z | |
dc.description | This is the final version. Available from Nature Research via the DOI in this record. | en_GB |
dc.identifier.journal | Scientific Reports | en_GB |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2019-06-20 | |
exeter.funder | ::Centre for Environment, Fisheries and Aquaculture Science (CEFAS) | en_GB |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2019-12-01 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2019-10-04T15:18:31Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2019-10-04T15:22:42Z | |
refterms.panel | A | en_GB |
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Except where otherwise noted, this item's licence is described as © The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre-ative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not per-mitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/