dc.contributor.author | Amanatidou, E | |
dc.contributor.author | Matthews, A | |
dc.contributor.author | Kuhlicke, U | |
dc.contributor.author | Neu, T | |
dc.contributor.author | McEvoy, J | |
dc.contributor.author | Raymond, B | |
dc.date.accessioned | 2019-10-21T10:42:51Z | |
dc.date.issued | 2019-10-21 | |
dc.description.abstract | Gram-negative bacteria such as Escherichia coli commonly resist β-lactam antibiotics using plasmid-encoded β-lactamase enzymes. Bacterial strains that express β-lactamases have been found to detoxify liquid cultures and thus to protect genetically susceptible strains, constituting a clear laboratory example of social protection. These results are not necessarily general; on solid media, for instance, the rapid bactericidal action of β-lactams largely prevents social protection. Here we tested the hypothesis that the greater tolerance of biofilm bacteria for β-lactams would facilitate social interactions. We used a recently isolated E. coli strain, capable of strong biofilm formation, to compare how cooperation and exploitation in colony biofilms and broth culture drives the dynamics of a non-conjugative plasmid encoding a clinically important β-lactamase. Susceptible cells in biofilms were tolerant of ampicillin - high doses and several days of exposure were required to kill them. In support of our hypothesis, we found robust social protection of susceptible E. coli in biofilms, despite fine-scale physical separation of resistant and susceptible cells and lower rates of production of extracellular β-lactamase. In contrast, social interactions in broth were restricted to a relatively narrow range of ampicillin doses. Our results show that β-lactam selection pressure on Gram-negative biofilms leads to cooperative resistance characterized by a low equilibrium frequency of resistance plasmids, sufficient to protect all cells. | en_GB |
dc.description.sponsorship | Medical Research Council (MRC) | en_GB |
dc.format | Data files are comprised of tab(.txt) or comma delimited (.csv) text with a supporting readme file ((.rtf) for the most complex data set describing the transfer experimentss | en_GB |
dc.identifier.doi | 10.24378/exe.1943 | |
dc.identifier.grantnumber | ES/N009797/1 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/39284 | |
dc.language.iso | en | en_GB |
dc.publisher | University of Exeter | en_GB |
dc.rights | CC BY 4.0 | en_GB |
dc.title | Biofilms facilitate cheating and social exploitation of β-lactam resistance in Escherichia coli (dataset) | en_GB |
dc.type | Dataset | en_GB |
dc.date.available | 2019-10-21T10:42:51Z | |
dc.description | This is the dataset used for the Amanatidou et al. (2019) article "Biofilms facilitate cheating and social exploitation of β-lactam resistance in Escherichia coli" published in npj Biofilms and Microbiomes. | en_GB |
dc.description | Raw data for figures 1, and 3 through 6, for accepted MS. No special software is required to view this views. Data files are comprised of tab or comma delimited text. | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0 | en_GB |
exeter.funder | ::Medical Research Council (MRC) | en_GB |
rioxxterms.version | NA | en_GB |
rioxxterms.type | Other | en_GB |
refterms.dateFOA | 2019-10-21T10:42:58Z | |