Intracellular Infection of Diverse Diatoms by an Evolutionary Distinct Relative of the Fungi
dc.contributor.author | Chambouvet, A | |
dc.contributor.author | Monier, A | |
dc.contributor.author | Maguire, F | |
dc.contributor.author | Itoïz, S | |
dc.contributor.author | del Campo, J | |
dc.contributor.author | Elies, P | |
dc.contributor.author | Edvardsen, B | |
dc.contributor.author | Eikreim, W | |
dc.contributor.author | Richards, TA | |
dc.date.accessioned | 2019-11-26T15:26:36Z | |
dc.date.issued | 2019-11-14 | |
dc.description.abstract | The Fungi are a diverse kingdom, dominating terrestrial environments and driving important ecologies. Although fungi, and the related Opisthosporidia, interact with photosynthetic organisms on land and in freshwater as parasites, symbionts, and/or saprotrophic degraders, such interactions in the marine environment are poorly understood. One newly identified uncultured marine lineage has been named novel chytrid-like-clade-1 (NCLC1) or basal-clone-group-I. We use ribosomal RNA (rRNA) encoding gene phylogenies to demonstrate that NCLC1 is a distinct branch within the Opisthosporidia (Holomycota). Opisthosporidia are a diverse and largely uncultured group that form a sister branch to the Fungi or, alternatively, the deepest branch within the Fungi, depending on how the boundary to this kingdom is inferred. Using culture-free lineage-specific rRNA-targeted fluorescent in situ hybridization (FISH) microscopy, we demonstrate that NCLC1 cells form intracellular infection of key diatom species, establishing that intracellular colonization of a eukaryotic host is a consistent lifestyle across the Opisthosporidia. NCLC1 infection-associated loss and/or envelopment of the diatom nuclei infers a necrotrophic-pathogenic interaction. Diatoms are one of the most diverse and ecologically important phytoplankton groups, acting as dominant primary producers and driving carbon fixation and storage in many aquatic environments. Our results provide insight into the diversity of microbial eukaryotes that interact with diatoms. We suggest that such interactions can play a key role in diatom associated ecosystem functions, such as the marine carbon pump through necrotrophic-parasitism, facilitating the export of diatoms to the sediment. | en_GB |
dc.description.sponsorship | Agence Nationale de la Recherche (ANR) | en_GB |
dc.description.sponsorship | Genome Canada | en_GB |
dc.description.sponsorship | Donald Hill Family Fellowship | en_GB |
dc.description.sponsorship | Royal Society | en_GB |
dc.description.sponsorship | BiodivERsA | en_GB |
dc.description.sponsorship | Gordon and Betty Moore Foundation | en_GB |
dc.identifier.citation | Published online 14 November 2019 | en_GB |
dc.identifier.doi | 10.1016/j.cub.2019.09.074 | |
dc.identifier.grantnumber | ANR-16_ACHN_0003 | en_GB |
dc.identifier.grantnumber | GBMF3307 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/39817 | |
dc.language.iso | en | en_GB |
dc.publisher | Elsevier (Cell Press) | en_GB |
dc.rights | © 2019 Crown Copyright © 2019 Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/). | en_GB |
dc.subject | parasite | en_GB |
dc.subject | Opisthosporidia | en_GB |
dc.subject | oceanic carbon storage | en_GB |
dc.subject | fungal phylogeny | en_GB |
dc.title | Intracellular Infection of Diverse Diatoms by an Evolutionary Distinct Relative of the Fungi | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2019-11-26T15:26:36Z | |
dc.identifier.issn | 0960-9822 | |
dc.description | This is the final version. Available on open access from Elsevier via the DOI in this record | en_GB |
dc.description | Data and Code Availability: All data and code are available with DOI’s given in the methods section. Specifically, physical and chemical parameters of the water column obtained using CTD ocean water sampling are available at http://biomarks.eu/ctd007 (and replicated here at figshare DOI: 10.6084/m9.figshare.9821936). The phylogenetic tree file, masked and unmasked SSU rDNA alignments are available at Zenodo repository: DOI 10.5281/zenodo.2788876. All sequence data used were derived from the NCBI ‘GenBank’ database and accession numbers are provided in Figure. 1B. The R code used to test statistical association in the FISH data are available at Zenodo repository: DOI 10.5281/zenodo.2788876. | en_GB |
dc.identifier.journal | Current Biology | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | en_GB |
dcterms.dateAccepted | 2019-09-30 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2019-11-14 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2019-11-26T15:21:46Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2019-11-26T15:26:40Z | |
refterms.panel | A | en_GB |
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This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/).