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dc.contributor.authorWideman, JG
dc.contributor.authorMonier, A
dc.contributor.authorRodríguez-Martínez, R
dc.contributor.authorLeonard, G
dc.contributor.authorCook, E
dc.contributor.authorPoirier, C
dc.contributor.authorMaguire, F
dc.contributor.authorMilner, DS
dc.contributor.authorIrwin, NAT
dc.contributor.authorMoore, K
dc.contributor.authorSantoro, AE
dc.contributor.authorKeeling, PJ
dc.contributor.authorWorden, AZ
dc.contributor.authorRichards, TA
dc.date.accessioned2019-11-26T15:53:36Z
dc.date.issued2019-11-25
dc.description.abstractMost eukaryotic microbial diversity is uncultivated, under-studied and lacks nuclear genome data. Mitochondrial genome sampling is more comprehensive, but many phylogenetically important groups remain unsampled. Here, using a single-cell sorting approach combining tubulin-specific labelling with photopigment exclusion, we sorted flagellated heterotrophic unicellular eukaryotes from Pacific Ocean samples. We recovered 206 single amplified genomes, predominantly from underrepresented branches on the tree of life. Seventy single amplified genomes contained unique mitochondrial contigs, including 21 complete or near-complete mitochondrial genomes from formerly under-sampled phylogenetic branches, including telonemids, katablepharids, cercozoans and marine stramenopiles, effectively doubling the number of available samples of heterotrophic flagellate mitochondrial genomes. Collectively, these data identify a dynamic history of mitochondrial genome evolution including intron gain and loss, extensive patterns of genetic code variation and complex patterns of gene loss. Surprisingly, we found that stramenopile mitochondrial content is highly plastic, resembling patterns of variation previously observed only in plants.en_GB
dc.description.sponsorshipGordon and Betty Moore Foundationen_GB
dc.description.sponsorshipLeverhulme Trusten_GB
dc.description.sponsorshipDavid and Lucile Packard Foundationen_GB
dc.description.sponsorshipRoyal Societyen_GB
dc.description.sponsorshipEuropean Molecular Biology Organizationen_GB
dc.description.sponsorshipCONICYT FONDECYTen_GB
dc.description.sponsorshipGenome Canadaen_GB
dc.identifier.citationPublished online 25 November 2019en_GB
dc.identifier.doi10.1038/s41564-019-0605-4
dc.identifier.grantnumberGBMF3307en_GB
dc.identifier.grantnumberPLP-2014–147en_GB
dc.identifier.grantnumberALTF 761–2014en_GB
dc.identifier.grantnumber11170748en_GB
dc.identifier.urihttp://hdl.handle.net/10871/39819
dc.language.isoenen_GB
dc.publisherNature Researchen_GB
dc.rights.embargoreasonUnder embargo until 25 May 2020 in compliance with publisher policyen_GB
dc.rights© The Author(s), under exclusive licence to Springer Nature Limited 2019en_GB
dc.titleUnexpected mitochondrial genome diversity revealed by targeted single-cell genomics of heterotrophic flagellated protistsen_GB
dc.typeArticleen_GB
dc.date.available2019-11-26T15:53:36Z
dc.descriptionThis is the author accepted manuscript. The final version is available from Nature Research via the DOI in this recorden_GB
dc.descriptionData availability: Complete mtDNA sequences assembled from this study are available at GenBank under the accession numbers MK188935 to MK188947, MN082144 and MN082145. Sequencing data are available under NCBI BioProject PRJNA379597. Reads have been deposited at NCBI Sequence Read Archive with accession number SRP102236. Partial mtDNA contigs and other important contigs mentioned in the text are available from Figshare at https://doi.org/10.6084/m9.figshare.7314728. Nuclear SAG assemblies are available from Figshare at https://doi.org/10.6084/m9.figshare.7352966. A protocol is available from protocols.io at: https://doi.org/10.17504/protocols.io.ywpfxdn.en_GB
dc.descriptionCode availability: The bioinformatic workflow is available at https://doi.org/10.5281/zenodo.192677; additional statistical analysis code is available at https://doi.org/10.6084/m9.figshare.9884309.en_GB
dc.identifier.eissn2058-5276
dc.identifier.journalNature Microbiologyen_GB
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserveden_GB
dcterms.dateAccepted2019-10-08
exeter.funder::Gordon and Betty Moore Foundationen_GB
exeter.funder::Leverhulme Trusten_GB
exeter.funder::Gordon and Betty Moore Foundationen_GB
exeter.funder::Gordon and Betty Moore Foundationen_GB
rioxxterms.versionAMen_GB
rioxxterms.licenseref.startdate2019-11-25
rioxxterms.typeJournal Article/Reviewen_GB
refterms.dateFCD2019-11-26T15:48:27Z
refterms.versionFCDAM
refterms.panelAen_GB


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