dc.contributor.author | Wideman, JG | |
dc.contributor.author | Monier, A | |
dc.contributor.author | Rodríguez-Martínez, R | |
dc.contributor.author | Leonard, G | |
dc.contributor.author | Cook, E | |
dc.contributor.author | Poirier, C | |
dc.contributor.author | Maguire, F | |
dc.contributor.author | Milner, DS | |
dc.contributor.author | Irwin, NAT | |
dc.contributor.author | Moore, K | |
dc.contributor.author | Santoro, AE | |
dc.contributor.author | Keeling, PJ | |
dc.contributor.author | Worden, AZ | |
dc.contributor.author | Richards, TA | |
dc.date.accessioned | 2019-11-26T15:53:36Z | |
dc.date.issued | 2019-11-25 | |
dc.description.abstract | Most eukaryotic microbial diversity is uncultivated, under-studied and lacks nuclear genome data. Mitochondrial genome sampling is more comprehensive, but many phylogenetically important groups remain unsampled. Here, using a single-cell sorting approach combining tubulin-specific labelling with photopigment exclusion, we sorted flagellated heterotrophic unicellular eukaryotes from Pacific Ocean samples. We recovered 206 single amplified genomes, predominantly from underrepresented branches on the tree of life. Seventy single amplified genomes contained unique mitochondrial contigs, including 21 complete or near-complete mitochondrial genomes from formerly under-sampled phylogenetic branches, including telonemids, katablepharids, cercozoans and marine stramenopiles, effectively doubling the number of available samples of heterotrophic flagellate mitochondrial genomes. Collectively, these data identify a dynamic history of mitochondrial genome evolution including intron gain and loss, extensive patterns of genetic code variation and complex patterns of gene loss. Surprisingly, we found that stramenopile mitochondrial content is highly plastic, resembling patterns of variation previously observed only in plants. | en_GB |
dc.description.sponsorship | Gordon and Betty Moore Foundation | en_GB |
dc.description.sponsorship | Leverhulme Trust | en_GB |
dc.description.sponsorship | David and Lucile Packard Foundation | en_GB |
dc.description.sponsorship | Royal Society | en_GB |
dc.description.sponsorship | European Molecular Biology Organization | en_GB |
dc.description.sponsorship | CONICYT FONDECYT | en_GB |
dc.description.sponsorship | Genome Canada | en_GB |
dc.identifier.citation | Published online 25 November 2019 | en_GB |
dc.identifier.doi | 10.1038/s41564-019-0605-4 | |
dc.identifier.grantnumber | GBMF3307 | en_GB |
dc.identifier.grantnumber | PLP-2014–147 | en_GB |
dc.identifier.grantnumber | ALTF 761–2014 | en_GB |
dc.identifier.grantnumber | 11170748 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/39819 | |
dc.language.iso | en | en_GB |
dc.publisher | Nature Research | en_GB |
dc.rights.embargoreason | Under embargo until 25 May 2020 in compliance with publisher policy | en_GB |
dc.rights | © The Author(s), under exclusive licence to Springer Nature Limited 2019 | en_GB |
dc.title | Unexpected mitochondrial genome diversity revealed by targeted single-cell genomics of heterotrophic flagellated protists | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2019-11-26T15:53:36Z | |
dc.description | This is the author accepted manuscript. The final version is available from Nature Research via the DOI in this record | en_GB |
dc.description | Data availability:
Complete mtDNA sequences assembled from this study are available at GenBank under the accession numbers MK188935 to MK188947, MN082144 and MN082145. Sequencing data are available under NCBI BioProject PRJNA379597. Reads have been deposited at NCBI Sequence Read Archive with accession number SRP102236. Partial mtDNA contigs and other important contigs mentioned in the text are available from Figshare at https://doi.org/10.6084/m9.figshare.7314728. Nuclear SAG assemblies are available from Figshare at https://doi.org/10.6084/m9.figshare.7352966. A protocol is available from protocols.io at: https://doi.org/10.17504/protocols.io.ywpfxdn. | en_GB |
dc.description | Code availability:
The bioinformatic workflow is available at https://doi.org/10.5281/zenodo.192677; additional statistical analysis code is available at https://doi.org/10.6084/m9.figshare.9884309. | en_GB |
dc.identifier.eissn | 2058-5276 | |
dc.identifier.journal | Nature Microbiology | en_GB |
dc.rights.uri | http://www.rioxx.net/licenses/all-rights-reserved | en_GB |
dcterms.dateAccepted | 2019-10-08 | |
exeter.funder | ::Gordon and Betty Moore Foundation | en_GB |
exeter.funder | ::Leverhulme Trust | en_GB |
exeter.funder | ::Gordon and Betty Moore Foundation | en_GB |
exeter.funder | ::Gordon and Betty Moore Foundation | en_GB |
rioxxterms.version | AM | en_GB |
rioxxterms.licenseref.startdate | 2019-11-25 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2019-11-26T15:48:27Z | |
refterms.versionFCD | AM | |
refterms.panel | A | en_GB |