dc.contributor.author | Pereira, L | |
dc.contributor.author | Bittencourt, PRL | |
dc.contributor.author | Pacheco, VS | |
dc.contributor.author | Miranda, MT | |
dc.contributor.author | Zhang, Y | |
dc.contributor.author | Oliveira, RS | |
dc.contributor.author | Groenendijk, P | |
dc.contributor.author | Machado, EC | |
dc.contributor.author | Tyree, MT | |
dc.contributor.author | Jansen, S | |
dc.contributor.author | Rowland, L | |
dc.contributor.author | Ribeiro, RV | |
dc.date.accessioned | 2019-11-27T11:20:21Z | |
dc.date.issued | 2019-08-28 | |
dc.description.abstract | Xylem vulnerability to embolism represents an important trait to determine species distribution patterns and drought resistance. However, estimating embolism resistance frequently requires time-consuming and ambiguous hydraulic lab measurements. Based on a recently developed pneumatic method, we present and test the “Pneumatron”, a device that generates high time-resolution and fully automated vulnerability curves. Embolism resistance is estimated by applying a partial vacuum to extract air from an excised xylem sample, while monitoring the pressure change over time. Although the amount of gas extracted is strongly correlated with the percentage loss of xylem conductivity, validation of the Pneumatron was performed by comparison with the optical method for Eucalyptus camaldulensis leaves. The Pneumatron improved the precision of the pneumatic method considerably, facilitating the detection of small differences in the (percentage of air discharged [PAD] < 0.47%). Hence, the Pneumatron can directly measure the 50% PAD without any fitting of vulnerability curves. PAD and embolism frequency based on the optical method were strongly correlated (r2 = 0.93) for E. camaldulensis. By providing an open source platform, the Pneumatron represents an easy, low-cost, and powerful tool for field measurements, which can significantly improve our understanding of plant–water relations and the mechanisms behind embolism. | en_GB |
dc.description.sponsorship | Natural Environment Research Council (NERC) | en_GB |
dc.description.sponsorship | Royal Society | en_GB |
dc.description.sponsorship | National Council for Scientific and Technological Development (CNPq, Brazil) | en_GB |
dc.description.sponsorship | São Paulo Research Foundation (FAPESP, Brazil) | en_GB |
dc.identifier.citation | Published online 28 August 2019 | en_GB |
dc.identifier.doi | 10.1111/pce.13647 | |
dc.identifier.grantnumber | NE/N014022/1 | en_GB |
dc.identifier.grantnumber | 401104/2016‐8 | en_GB |
dc.identifier.grantnumber | 2018/09834‐5 | en_GB |
dc.identifier.grantnumber | 2018/01847‐0 | en_GB |
dc.identifier.grantnumber | 2019/07773‐1 | en_GB |
dc.identifier.grantnumber | 2017/14075‐3 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/39844 | |
dc.language.iso | en | en_GB |
dc.publisher | Wiley | en_GB |
dc.rights.embargoreason | Under embargo until 28 August 2020 in compliance with publisher policy | en_GB |
dc.rights | © 2019 John Wiley & Sons Ltd | en_GB |
dc.subject | pneumatic method | en_GB |
dc.subject | vulnerability curves | en_GB |
dc.subject | cavitation | en_GB |
dc.subject | drought resistance | en_GB |
dc.subject | water transport | en_GB |
dc.subject | plant hydraulics | en_GB |
dc.title | The Pneumatron: An automated pneumatic apparatus for estimating xylem vulnerability to embolism at high temporal resolution | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2019-11-27T11:20:21Z | |
dc.identifier.issn | 0140-7791 | |
dc.description | This is the author accepted manuscript. The final version is available from Wiley via the DOI in this record | en_GB |
dc.identifier.journal | Plant Cell and Environment | en_GB |
dc.rights.uri | http://www.rioxx.net/licenses/all-rights-reserved | en_GB |
dcterms.dateAccepted | 2019-08-20 | |
exeter.funder | ::Natural Environment Research Council (NERC) | en_GB |
exeter.funder | ::Royal Society (Government) | en_GB |
rioxxterms.version | AM | en_GB |
rioxxterms.licenseref.startdate | 2019-08-28 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2019-11-27T11:17:09Z | |
refterms.versionFCD | AM | |
refterms.panel | C | en_GB |