Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration
dc.contributor.author | Zivanovic, J | |
dc.contributor.author | Kouroussis, E | |
dc.contributor.author | Kohl, JB | |
dc.contributor.author | Adhikari, B | |
dc.contributor.author | Bursac, B | |
dc.contributor.author | Schott-Roux, S | |
dc.contributor.author | Petrovic, D | |
dc.contributor.author | Miljkovic, JL | |
dc.contributor.author | Thomas-Lopez, D | |
dc.contributor.author | Jung, Y | |
dc.contributor.author | Miler, M | |
dc.contributor.author | Mitchell, S | |
dc.contributor.author | Milosevic, V | |
dc.contributor.author | Gomes, JE | |
dc.contributor.author | Benhar, M | |
dc.contributor.author | Gonzales-Zorn, B | |
dc.contributor.author | Ivanovic-Burmazovic, I | |
dc.contributor.author | Torregrossa, R | |
dc.contributor.author | Mitchell, JR | |
dc.contributor.author | Whiteman, M | |
dc.contributor.author | Schwarz, G | |
dc.contributor.author | Snyder, SH | |
dc.contributor.author | Paul, BD | |
dc.contributor.author | Carroll, KS | |
dc.contributor.author | Filipovic, MR | |
dc.date.accessioned | 2020-01-23T10:21:41Z | |
dc.date.issued | 2019-11-14 | |
dc.description.abstract | Life on Earth emerged in a hydrogen sulfide (H2S)-rich environment eons ago and with it protein persulfidation mediated by H2S evolved as a signaling mechanism. Protein persulfidation (S-sulfhydration) is a post-translational modification of reactive cysteine residues, which modulate protein structure and/or function. Persulfides are difficult to label and study due to their reactivity and similarity with cysteine. Here, we report a facile strategy for chemoselective persulfide bioconjugation using dimedone-based probes, to achieve highly selective, rapid, and robust persulfide labeling in biological samples with broad utility. Using this method, we show persulfidation is an evolutionarily conserved modification and waves of persulfidation are employed by cells to resolve sulfenylation and prevent irreversible cysteine overoxidation preserving protein function. We report an age-associated decline in persulfidation that is conserved across evolutionary boundaries. Accordingly, dietary or pharmacological interventions to increase persulfidation associate with increased longevity and improved capacity to cope with stress stimuli. | en_GB |
dc.description.sponsorship | IDEX Bordeaux | en_GB |
dc.description.sponsorship | FRM | en_GB |
dc.description.sponsorship | Medical Research Council (MRC) | en_GB |
dc.description.sponsorship | Brian Ridge Scholarship | en_GB |
dc.description.sponsorship | Northcott Devon Medical Research Foundation | en_GB |
dc.description.sponsorship | Ministry of Education, Science and Technology Development of the Republic of Serbia | en_GB |
dc.description.sponsorship | NIH | en_GB |
dc.description.sponsorship | DFG, Germany | en_GB |
dc.description.sponsorship | American Heart Association-Allen Initiative in Brain Health and Cognitive Impairment | en_GB |
dc.identifier.citation | Vol. 30 (6), pp. 1152 - 1170.e13 | en_GB |
dc.identifier.doi | 10.1016/j.cmet.2019.10.007 | |
dc.identifier.grantnumber | ANR-10-IDEX-03-02 | en_GB |
dc.identifier.grantnumber | DEQ20180339181 | en_GB |
dc.identifier.grantnumber | MR/M022706/1 | en_GB |
dc.identifier.grantnumber | 173009 | en_GB |
dc.identifier.grantnumber | GM102187 | en_GB |
dc.identifier.grantnumber | SFB1218 TP B08 | en_GB |
dc.identifier.grantnumber | 19PABH134580006 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/40541 | |
dc.language.iso | en | en_GB |
dc.publisher | Cell Press | en_GB |
dc.relation.url | https://data.mendeley.com/datasets/pw2wz39tsk/2 | en_GB |
dc.relation.url | https://doi.org/10.1016/j.cmet.2019.12.001 | |
dc.rights.embargoreason | Under embargo until 3 December 2020 in compliance with publisher policy | en_GB |
dc.rights | © 2019. This version is made available under the CC-BY-NC-ND 4.0 license: https://creativecommons.org/licenses/by-nc-nd/4.0/ | en_GB |
dc.subject | hydrogen sulfide | en_GB |
dc.subject | protein persulfidation | en_GB |
dc.subject | hydrogen peroxide | en_GB |
dc.subject | sulfenylation | en_GB |
dc.subject | sulfinylation | en_GB |
dc.subject | sulfonylation | en_GB |
dc.subject | redox signaling | en_GB |
dc.subject | aging | en_GB |
dc.subject | calorie restriction | en_GB |
dc.title | Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2020-01-23T10:21:41Z | |
dc.identifier.issn | 1550-4131 | |
dc.description | This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this record | en_GB |
dc.description | Data and Code Availability: The raw data corresponding to the antibody microarray Detection of persulfidation of EGFR Pathway kinases represent entirely new application of the dimedone switch method and are therefore available in more detail from the corresponding author on request. Proteomic data used for Figure S2C are stored in Data S1 and S2 and for Figures S3H and S3I in Data S3 and S4. All in-gel persulfidation, as well as Western blot sufenylation and sulfynilation data are reported in main and supporting Figures. Other raw data are available at https://data.mendeley.com/datasets/pw2wz39tsk/2 | en_GB |
dc.description | The following correction was published in Cell Metabolism Vol 31 (1), p. 207 (7 January 2020). DOI: https://doi.org/10.1016/j.cmet.2019.12.001. "As a result of an author oversight in the originally published version of this article, the surname of author Bruno Gonzalez-Zorn was misspelled as “Gonzales-Zorn.” Additionally, the scheme in the Graphical Abstract contains a final product of protein-S-S-dimedone, rather than protein-S-dimedone. These errors have now been corrected in the article online. The authors apologize for the errors and any inconvenience that may have resulted." | |
dc.identifier.journal | Cell Metabolism | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | en_GB |
dcterms.dateAccepted | 2019-10-18 | |
exeter.funder | ::Medical Research Council (MRC) | en_GB |
rioxxterms.version | AM | en_GB |
rioxxterms.licenseref.startdate | 2019-11-14 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2020-01-23T10:13:24Z | |
refterms.versionFCD | AM | |
refterms.dateFOA | 2020-12-03T00:00:00Z | |
refterms.panel | A | en_GB |
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Except where otherwise noted, this item's licence is described as © 2019. This version is made available under the CC-BY-NC-ND 4.0 license: https://creativecommons.org/licenses/by-nc-nd/4.0/