Notch signaling coordinates ommatidial rotation in the Drosophila eye via transcriptional regulation of the EGF-Receptor ligand Argos
dc.contributor.author | Koca, Y | |
dc.contributor.author | Housden, BE | |
dc.contributor.author | Gault, WJ | |
dc.contributor.author | Bray, SJ | |
dc.contributor.author | Mlodzik, M | |
dc.date.accessioned | 2020-01-24T10:52:20Z | |
dc.date.issued | 2019-12-09 | |
dc.description.abstract | In all metazoans, a small number of evolutionarily conserved signaling pathways are reiteratively used during development to orchestrate critical patterning and morphogenetic processes. Among these, Notch (N) signaling is essential for most aspects of tissue patterning where it mediates the communication between adjacent cells to control cell fate specification. In Drosophila, Notch signaling is required for several features of eye development, including the R3/R4 cell fate choice and R7 specification. Here we show that hypomorphic alleles of Notch, belonging to the Nfacet class, reveal a novel phenotype: while photoreceptor specification in the mutant ommatidia is largely normal, defects are observed in ommatidial rotation (OR), a planar cell polarity (PCP)-mediated cell motility process. We demonstrate that during OR Notch signaling is specifically required in the R4 photoreceptor to upregulate the transcription of argos (aos), an inhibitory ligand to the epidermal growth factor receptor (EGFR), to fine-tune the activity of EGFR signaling. Consistently, the loss-of-function defects of Nfacet alleles and EGFR-signaling pathway mutants are largely indistinguishable. A Notch-regulated aos enhancer confers R4 specific expression arguing that aos is directly regulated by Notch signaling in this context via Su(H)-Mam-dependent transcription. | en_GB |
dc.description.sponsorship | NIH/NEI | en_GB |
dc.identifier.citation | Vol. 9, Article number 18628 | en_GB |
dc.identifier.doi | 10.1038/s41598-019-55203-w | |
dc.identifier.grantnumber | RO1 EY13256 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/40559 | |
dc.language.iso | en | en_GB |
dc.publisher | Nature Research (Springer Nature) | en_GB |
dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | en_GB |
dc.subject | Cell signalling | en_GB |
dc.subject | Morphogenesis | en_GB |
dc.title | Notch signaling coordinates ommatidial rotation in the Drosophila eye via transcriptional regulation of the EGF-Receptor ligand Argos | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2020-01-24T10:52:20Z | |
exeter.article-number | 18628 | en_GB |
dc.description | This is the author accepted manuscript. | en_GB |
dc.identifier.eissn | 2045-2322 | |
dc.identifier.journal | Scientific Reports | en_GB |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2019-11-24 | |
rioxxterms.version | AM | en_GB |
rioxxterms.licenseref.startdate | 2019-11-24 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2020-01-24T10:48:44Z | |
refterms.versionFCD | AM | |
refterms.dateFOA | 2020-01-24T10:52:40Z | |
refterms.panel | A | en_GB |
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