Controlled sampling of ribosomally active protistan diversity in sediment-surface layers identifies putative players in the marine carbon sink
dc.contributor.author | Rodríguez-Martínez, R | |
dc.contributor.author | Leonard, G | |
dc.contributor.author | Milner, DS | |
dc.contributor.author | Sudek, S | |
dc.contributor.author | Conway, M | |
dc.contributor.author | Moore, K | |
dc.contributor.author | Hudson, T | |
dc.contributor.author | Mahé, F | |
dc.contributor.author | Keeling, PJ | |
dc.contributor.author | Santoro, AE | |
dc.contributor.author | Worden, AZ | |
dc.contributor.author | Richards, TA | |
dc.date.accessioned | 2020-02-05T14:24:54Z | |
dc.date.issued | 2020-01-01 | |
dc.description.abstract | Marine sediments are one of the largest carbon reservoir on Earth, yet the microbial communities, especially the eukaryotes, that drive these ecosystems are poorly characterised. Here, we report implementation of a sampling system that enables injection of reagents into sediments at depth, allowing for preservation of RNA in situ. Using the RNA templates recovered, we investigate the ‘ribosomally active’ eukaryotic diversity present in sediments close to the water/sediment interface. We demonstrate that in situ preservation leads to recovery of a significantly altered community profile. Using SSU rRNA amplicon sequencing, we investigated the community structure in these environments, demonstrating a wide diversity and high relative abundance of stramenopiles and alveolates, specifically: Bacillariophyta (diatoms), labyrinthulomycetes and ciliates. The identification of abundant diatom rRNA molecules is consistent with microscopy-based studies, but demonstrates that these algae can also be exported to the sediment as active cells as opposed to dead forms. We also observe many groups that include, or branch close to, osmotrophic–saprotrophic protists (e.g. labyrinthulomycetes and Pseudofungi), microbes likely to be important for detrital decomposition. The sequence data also included a diversity of abundant amplicon-types that branch close to the Fonticula slime moulds. Taken together, our data identifies additional roles for eukaryotic microbes in the marine carbon cycle; where putative osmotrophic–saprotrophic protists represent a significant active microbial-constituent of the upper sediment layer. | en_GB |
dc.description.sponsorship | Gordon and Betty Moore foundation | en_GB |
dc.description.sponsorship | Philip Leverhulme Award | en_GB |
dc.description.sponsorship | David and Lucile Packard Foundation | en_GB |
dc.description.sponsorship | CONICYT FONDECYT | en_GB |
dc.description.sponsorship | MBARI | en_GB |
dc.identifier.citation | Published online 09 January 2020 | en_GB |
dc.identifier.doi | 10.1038/s41396-019-0581-y | |
dc.identifier.grantnumber | GBMF3307 | en_GB |
dc.identifier.grantnumber | PLP-2014-147 | en_GB |
dc.identifier.grantnumber | 11170748. | en_GB |
dc.identifier.grantnumber | RG090623 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/40747 | |
dc.language.iso | en | en_GB |
dc.publisher | Springer | en_GB |
dc.rights | © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | en_GB |
dc.title | Controlled sampling of ribosomally active protistan diversity in sediment-surface layers identifies putative players in the marine carbon sink | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2020-02-05T14:24:54Z | |
dc.identifier.issn | 1751-7362 | |
dc.description | This is the final version. Available on open access from Springer via the DOI in this record. | en_GB |
dc.identifier.journal | ISME Journal | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2019-12-17 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2019-12-17 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2020-02-05T14:17:42Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2020-02-05T14:25:01Z | |
refterms.panel | A | en_GB |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's licence is described as © The Author(s) 2020.
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/