A Burkholderia pseudomallei macrophage infectivity potentiator-like protein has rapamycin-inhibitable peptidylprolyl isomerase activity and pleiotropic effects on virulence
Brown, Katherine A.
Titball, Richard W.
Infection and Immunity
American Society for Microbiology
Macrophage infectivity potentiators (Mips) are a group of virulence factors encoded by pathogenic bacteria such as Legionella, Chlamydia, and Neisseria species. Mips are part of the FK506-binding protein (FKBP) family, whose members typically exhibit peptidylprolyl cis-trans isomerase (PPIase) activity which is inhibitable by the immunosuppressants FK506 and rapamycin. Here we describe the identification and characterization of BPSS1823, a Mip-like protein in the intracellular pathogen Burkholderia pseudomallei. Recombinant BPSS1823 protein has rapamycin-inhibitable PPIase activity, indicating that it is a functional FKBP. A mutant strain generated by deletion of BPSS1823 in B. pseudomallei exhibited a reduced ability to survive within cells and significant attenuation in vivo, suggesting that BPSS1823 is important for B. pseudomallei virulence. In addition, pleiotropic effects were observed with a reduction in virulence mechanisms, including resistance to host killing mechanisms, swarming motility, and protease production.
addresses: Defence Science and Technology Laboratory, Porton Down SP4 0JQ, UK. firstname.lastname@example.org
notes: PMCID: PMC3257933
types: Journal Article; Research Support, Non-U.S. Gov't
This is the author's post-print version of an article published in Infection and Immunity , 2011, Vol. 79, Issue 11, pp. 4299 – 4307. Copyright © 2011, American Society for Microbiology
Infection and Immunity , 2011, Vol. 79, Issue 11, pp. 4299 - 4307
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