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dc.contributor.authorMansfield, Jessicaen_GB
dc.contributor.authorWinlove, C. Peteren_GB
dc.contributor.authorMoger, Julianen_GB
dc.contributor.authorMatcher, Steve J.en_GB
dc.date.accessioned2013-03-15T15:49:49Zen_GB
dc.date.accessioned2013-03-20T13:24:12Z
dc.date.issued2008-07-01en_GB
dc.description.abstractSecond harmonic generation (SHG) and two-photon fluorescence (TPF) microscopy is used to image the intercellular and pericellular matrix in normal and degenerate equine articular cartilage. The polarization sensitivity of SHG can be used directly to determine fiber orientation in the superficial 10 to 20 microm of tissue, and images of the ratio of intensities taken with two orthogonal polarization states reveal small scale variations in the collagen fiber organization that have not previously been reported. The signal from greater depths is influenced by the birefringence and biattenuance of the overlying tissue. An assessment of these effects is developed, based on the analysis of changes in TPF polarization with depth, and the approach is validated in tendon where composition is independent of depth. The analysis places an upper bound on the biattenuance of tendon of 2.65 x 10(-4). Normal cartilage reveals a consistent pattern of variation in fibril orientation with depth. In lesions, the pattern is severely disrupted and there are changes in the pericellular matrix, even at the periphery where the tissue appears microscopically normal. Quantification of polarization sensitivity changes with depth in cartilage will require detailed numerical models, but in the meantime, multiphoton microscopy provides sensitive indications of matrix changes in cartilage degeneration.en_GB
dc.identifier.citationVol. 13 (4), article 044020en_GB
dc.identifier.doi10.1117/1.2950318en_GB
dc.identifier.urihttp://hdl.handle.net/10036/4485en_GB
dc.language.isoenen_GB
dc.publisherSociety of Photo-optical Instrumentation Engineers (SPIE)en_GB
dc.subjectcartilageen_GB
dc.subjecttwo-photon fluorescence microscopyen_GB
dc.subjectsecond-harmonic microscopyen_GB
dc.subjectextracellular matrixen_GB
dc.subjectmultiphoton microscopyen_GB
dc.titleCollagen fiber arrangement in normal and diseased cartilage studied by polarization sensitive nonlinear microscopyen_GB
dc.typeArticleen_GB
dc.date.available2013-03-15T15:49:49Zen_GB
dc.date.available2013-03-20T13:24:12Z
dc.identifier.issn1083-3668en_GB
exeter.place-of-publicationUnited Statesen_GB
dc.descriptionJessica C. Mansfield ; C. Peter Winlove ; Julian Moger and Steve J. Matcher "Collagen fiber arrangement in normal and diseased cartilage studied by polarization sensitive nonlinear microscopy", J. Biomed. Opt. 13(4), 044020 (July 15, 2008). Copyright © 2008 Society of Photo-Optical Instrumentation Engineersen_GB
dc.identifier.eissn1560-2281en_GB
dc.identifier.journalJournal of Biomedical Opticsen_GB


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