Analysis of known bacterial protein vaccine antigens reveals biased physical properties and amino acid composition
Titball, Richard W.
University of Exeter (at the time of publication Richard Titball was at the Defence, Science and Technology Laboratory, Salisbury, UK); Defence, Science and Technology Laboratory
Comparative and Functional Genomics
Hindawi Publishing Corporation
Many vaccines have been developed from live attenuated forms of bacterial pathogens or from killed bacterial cells. However, an increased awareness of the potential for transient side-effects following vaccination has prompted an increased emphasis on the use of sub-unit vaccines, rather than those based on whole bacterial cells. The identification of vaccine sub-units is often a lengthy process and bioinformatics approaches have recently been used to identify candidate protein vaccine antigens. Such methods ultimately offer the promise of a more rapid advance towards preclinical studies with vaccines. We have compared the properties of known bacterial vaccine antigens against randomly selected proteins and identified differences in the make-up of these two groups. A computer algorithm that exploits these differences allows the identification of potential vaccine antigen candidates from pathogenic bacteria on the basis of their amino acid composition, a property inherently associated with sub-cellular location.
Copyright © 2003 John Wiley & Sons, Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
4 (5), pp. 468-478