Optical Interrogation of Sympathetic Neuronal Effects on Macroscopic Cardiomyocyte Network Dynamics
dc.contributor.author | Burton, R-AB | |
dc.contributor.author | Tomek, J | |
dc.contributor.author | Ambrosi, CM | |
dc.contributor.author | Larsen, HE | |
dc.contributor.author | Sharkey, AR | |
dc.contributor.author | Capel, RA | |
dc.contributor.author | Bilton, S | |
dc.contributor.author | Klimas, A | |
dc.contributor.author | Stephens, G | |
dc.contributor.author | Li, D | |
dc.contributor.author | Gallone, G | |
dc.contributor.author | Herring, N | |
dc.contributor.author | Mann, E | |
dc.contributor.author | Kumar, A | |
dc.contributor.author | Kramer, H | |
dc.contributor.author | Entcheva, E | |
dc.contributor.author | Paterson, DJ | |
dc.contributor.author | Bub, G | |
dc.date.accessioned | 2020-07-14T14:19:34Z | |
dc.date.issued | 2020-07-01 | |
dc.description.abstract | Cardiac stimulation via sympathetic neurons can potentially trigger arrhythmias. We present approaches to study neuron-cardiomyocyte interactions involving optogenetic selective probing and all-optical electrophysiology to measure activity in an automated fashion. Here we demonstrate the utility of optical interrogation of sympathetic neurons and their effects on macroscopic cardiomyocyte network dynamics to address research targets such as the effects of adrenergic stimulation via the release of neurotransmitters, the effect of neuronal numbers on cardiac behavior, and the applicability of optogenetics in mechanistic in vitro studies. As arrhythmias are emergent behaviors that involve the coordinated activity of millions of cells, we image at macroscopic scales to capture complex dynamics. We show that neurons can both decrease and increase wave stability and re-entrant activity in culture depending on their induced activity—a finding that may help us understand the often conflicting results seen in experimental and clinical studies. | en_GB |
dc.description.sponsorship | BHF Centre of Research Excellence, Oxford | en_GB |
dc.description.sponsorship | Engineering and Physical Sciences Research Council (EPSRC) | en_GB |
dc.description.sponsorship | Wellcome Trust | en_GB |
dc.description.sponsorship | Royal Society | en_GB |
dc.identifier.citation | Vol. 23 (7), article 101334 | en_GB |
dc.identifier.doi | 10.1016/j.isci.2020.101334 | |
dc.identifier.grantnumber | RE/18/3/34214 | en_GB |
dc.identifier.grantnumber | PG/11/6/28660 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/121934 | |
dc.language.iso | en | en_GB |
dc.publisher | Cell Press | en_GB |
dc.rights | © 2020 The Authors.This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) | en_GB |
dc.title | Optical Interrogation of Sympathetic Neuronal Effects on Macroscopic Cardiomyocyte Network Dynamics | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2020-07-14T14:19:34Z | |
dc.description | This is the final version. Available on open access from Cell Press via the DOI in this record | en_GB |
dc.identifier.eissn | 2589-0042 | |
dc.identifier.journal | iScience | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2020-06-26 | |
exeter.funder | ::Engineering and Physical Sciences Research Council (EPSRC) | en_GB |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2020-06-26 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2020-07-14T14:16:01Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2020-07-14T14:19:40Z | |
refterms.panel | B | en_GB |
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Except where otherwise noted, this item's licence is described as © 2020 The Authors.This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)