Single cell label-free probing of chromatin dynamics during B lymphocyte maturation
dc.contributor.author | Morrish, RB | |
dc.contributor.author | Yim, KH | |
dc.contributor.author | Pagliara, S | |
dc.contributor.author | Palombo, F | |
dc.contributor.author | Chahwan, R | |
dc.contributor.author | Stone, N | |
dc.date.accessioned | 2021-03-09T09:07:55Z | |
dc.date.issued | 2021-03-26 | |
dc.description.abstract | Large-scale intracellular signalling during developmental growth or in response to environmental alterations are largely orchestrated by chromatin within the cell nuclei. Chemical and conformational modifications of the chromatin architecture are critical steps in the regulation of differential gene expression and ultimately cell fate determination. Therefore, establishing chemical properties of the nucleus could provide key markers for phenotypic characterisation of cellular processes on a scale of individual cells. Raman microscopy is a sensitive technique that is capable of probing single cell chemical composition - and sub-cellular regions - in a label-free optical manner. As such, it has great potential in both clinical and basic research. However, perceived limitations of Raman spectroscopy such as low signal intensity and the difficulty in linking alterations in vibrational signals directly with ensuing biological effects have hampered advances in the field. Here we use immune B lymphocyte development as a model to assess chromatin and transcriptional changes using confocal Raman microscopy in combination with microfluidic devices and correlative transcriptomics, thereby linking changes in chemical and structural properties to biological outcomes. Live B lymphocytes were assessed before and after maturation. Multivariate analysis was applied to distinguish cellular components within each cell. The spectral differences between non-activated and activated B lymphocytes were then identified, and their correlation with known intracellular biological changes were assessed in comparison to conventional RNA-seq analysis. Our data shows that spectral analysis provides a powerful tool to study gene activation that can complement conventional molecular biology techniques and opens the way for mapping the dynamics in the biochemical makeup of individual cells. | en_GB |
dc.description.sponsorship | Engineering and Physical Sciences Research Council (EPSRC) | en_GB |
dc.description.sponsorship | Biotechnology and Biological Sciences Research Council (BBSRC) | en_GB |
dc.description.sponsorship | SNF | en_GB |
dc.description.sponsorship | Rosetrees Trust Fund | en_GB |
dc.description.sponsorship | UZH Research Priority Program | en_GB |
dc.description.sponsorship | RGS | en_GB |
dc.description.sponsorship | Medical Research Council (MRC) | en_GB |
dc.identifier.citation | Vol. 9, article 646616 | en_GB |
dc.identifier.doi | 10.3389/fcell.2021.646616 | |
dc.identifier.grantnumber | EP/M506527/1 | en_GB |
dc.identifier.grantnumber | BB/N017773/2 | en_GB |
dc.identifier.grantnumber | CRSK3_190550 | en_GB |
dc.identifier.grantnumber | M713 | en_GB |
dc.identifier.grantnumber | R2\180007 | en_GB |
dc.identifier.grantnumber | MC_PC_17189 to SP | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/125067 | |
dc.language.iso | en | en_GB |
dc.publisher | Frontiers Media | en_GB |
dc.relation.url | https://doi.org/10.6084/m9.figshare.14135219.v1 | en_GB |
dc.rights | © 2021 Morrish, Yim, Pagliara, Palombo, Chahwan and Stone. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | en_GB |
dc.subject | B cell | en_GB |
dc.subject | auxeticity | en_GB |
dc.subject | nuclear architecture | en_GB |
dc.subject | chromatin | en_GB |
dc.subject | vibrational spectroscopy | en_GB |
dc.subject | microfluidics | en_GB |
dc.title | Single cell label-free probing of chromatin dynamics during B lymphocyte maturation | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2021-03-09T09:07:55Z | |
dc.identifier.issn | 2296-634X | |
dc.description | This is the final version. Available on open access from Frontiers Media via the DOI in this record | en_GB |
dc.description | Data availability: The original contributions presented in the study are publicly available. This data can be found here: https://doi.org/10.6084/m9.figshare.14135219.v1 | en_GB |
dc.identifier.journal | Frontiers in Cell and Developmental Biology | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2021-03-08 | |
exeter.funder | ::Engineering and Physical Sciences Research Council (EPSRC) | en_GB |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2021-03-08 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2021-03-08T14:54:40Z | |
refterms.versionFCD | AM | |
refterms.dateFOA | 2021-03-26T11:15:48Z | |
refterms.panel | B | en_GB |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's licence is described as © 2021 Morrish, Yim, Pagliara, Palombo, Chahwan and Stone. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.