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dc.contributor.authorEerqing, N
dc.contributor.authorSubramanian, S
dc.contributor.authorRubio, J
dc.contributor.authorLutz, T
dc.contributor.authorWu, H-Y
dc.contributor.authorAnders, J
dc.contributor.authorSoeller, C
dc.contributor.authorVollmer, F
dc.date.accessioned2021-09-07T08:44:24Z
dc.date.issued2021-09-08
dc.description.abstractWe report a comparison of two photonic techniques for single-molecule sensing: fluorescence nanoscopy and optoplasmonic sensing. As the test system, oligonucleotides with and without fluorescent labels are transiently hybridized to complementary “docking” strands attached to gold nanorods. Comparing the measured single-molecule kinetics helps to examine the influence of the fluorescent labels as well as factors arising from different sensing geometries. Our results demonstrate that DNA dissociation is not significantly altered by the fluorescent labels and that DNA association is affected by geometric factors in the two techniques. These findings open the door to exploiting plasmonic sensing and fluorescence nanoscopy in a complementary fashion, which will aid in building more powerful sensors and uncovering the intricate effects that influence the behavior of single molecules.en_GB
dc.description.sponsorshipEngineering and Physical Sciences Research Council (EPSRC)en_GB
dc.description.sponsorshipRoyal Societyen_GB
dc.identifier.citationPublished online 8 September 2021en_GB
dc.identifier.doi10.1021/acsphotonics.1c01179
dc.identifier.grantnumberEP/L015331/1en_GB
dc.identifier.grantnumberEP/R031428/1en_GB
dc.identifier.grantnumberEP/T002875/1en_GB
dc.identifier.grantnumberEP/R045577/1en_GB
dc.identifier.grantnumberEP/N008235/1en_GB
dc.identifier.urihttp://hdl.handle.net/10871/126988
dc.language.isoenen_GB
dc.publisherAmerican Chemical Societyen_GB
dc.rights.embargoreasonUnder embargo until 8 September 2022 in compliance with publisher policyen_GB
dc.rights© 2021 American Chemical Society
dc.subjectsingle-moleculeen_GB
dc.subjectplasmonicsen_GB
dc.subjectwhispering gallery modesen_GB
dc.subjectoptoplasmonicen_GB
dc.subjectDNA-PAINTen_GB
dc.subjectfluorescenceen_GB
dc.subjectlocalization microscopyen_GB
dc.titleComparing transient oligonucleotide hybridization kinetics using DNA-PAINT and optoplasmonic single-molecule sensing on gold nanorodsen_GB
dc.typeArticleen_GB
dc.date.available2021-09-07T08:44:24Z
dc.identifier.issn2330-4022
dc.descriptionThis is the author accepted manuscript. The final version is available from the American Chemical Society via the DOI in this recorden_GB
dc.identifier.journalACS Photonicsen_GB
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserveden_GB
dcterms.dateAccepted2021-09-02
exeter.funder::Operating Budgeten_GB
exeter.funder::Engineering and Physical Sciences Research Council (EPSRC)en_GB
exeter.funder::Engineering and Physical Sciences Research Council (EPSRC)en_GB
rioxxterms.versionAMen_GB
rioxxterms.licenseref.startdate2021-09-02
rioxxterms.typeJournal Article/Reviewen_GB
refterms.dateFCD2021-09-06T19:24:05Z
refterms.versionFCDAM
refterms.panelBen_GB


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