S-nitrosothiols, and other products of nitrate metabolism, are increased in multiple human blood compartments following ingestion of beetroot juice
dc.contributor.author | Abu-Alghayth, M | |
dc.contributor.author | Vanhatalo, A | |
dc.contributor.author | Wylie, LJ | |
dc.contributor.author | McDonagh, ST | |
dc.contributor.author | Thompson, C | |
dc.contributor.author | Kadach, S | |
dc.contributor.author | Kerr, P | |
dc.contributor.author | Smallwood, MJ | |
dc.contributor.author | Jones, AM | |
dc.contributor.author | Winyard, PG | |
dc.date.accessioned | 2021-10-27T14:34:03Z | |
dc.date.issued | 2021-04-16 | |
dc.description.abstract | Ingested inorganic nitrate (NO3⁻) has multiple effects in the human body including vasodilation, inhibition of platelet aggregation, and improved skeletal muscle function. The functional effects of oral NO3⁻ involve the in vivo reduction of NO3⁻ to nitrite (NO2⁻) and thence to nitric oxide (NO). However, the potential involvement of S-nitrosothiol (RSNO) formation is unclear. We hypothesised that the RSNO concentration ([RSNO]) in red blood cells (RBCs) and plasma is increased by NO3⁻-rich beetroot juice ingestion. In healthy human volunteers, we tested the effect of dietary supplementation with NO3⁻-rich beetroot juice (BR) or NO3⁻-depleted beetroot juice (placebo; PL) on [RSNO], [NO3⁻] and [NO2⁻] in RBCs, whole blood and plasma, as measured by ozone-based chemiluminescence. The median basal [RSNO] in plasma samples (n = 22) was 10 (5–13) nM (interquartile range in brackets). In comparison, the median values for basal [RSNO] in the corresponding RBC preparations (n = 19) and whole blood samples (n = 19) were higher (p < 0.001) than in plasma, being 40 (30–60) nM and 35 (25–80) nM, respectively. The median RBC [RSNO] in a separate cohort of healthy subjects (n = 5) was increased to 110 (93–125) nM after ingesting BR (12.8 mmol NO3⁻) compared to a corresponding baseline value of 25 (21–31) nM (Mann-Whitney test, p < 0.01). The median plasma [RSNO] in another cohort of healthy subjects (n = 14) was increased almost ten-fold to 104 (58–151) nM after BR supplementation (7 × 6.4 mmol of NO3⁻ over two days, p < 0.01) compared to PL. In conclusion, RBC and plasma [RSNO] are increased by BR ingestion. In addition to NO2⁻, RSNO may be involved in dietary NO3⁻ metabolism/actions. | en_GB |
dc.description.sponsorship | University of Bisha (Saudi Arabia) | en_GB |
dc.description.sponsorship | Royal Embassy of the Saudi Arabia Cultural Bureau in the UK | en_GB |
dc.identifier.citation | Vol. 43, article 101974 | en_GB |
dc.identifier.doi | 10.1016/j.redox.2021.101974 | |
dc.identifier.uri | http://hdl.handle.net/10871/127600 | |
dc.language.iso | en | en_GB |
dc.publisher | Elsevier | en_GB |
dc.rights | © 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) | en_GB |
dc.subject | Nitrate | en_GB |
dc.subject | Nitrite | en_GB |
dc.subject | S-nitrosothiols (RSNO) | en_GB |
dc.subject | Plasma | en_GB |
dc.subject | Red blood cells (RBCs) | en_GB |
dc.subject | Beetroot (BR) | en_GB |
dc.title | S-nitrosothiols, and other products of nitrate metabolism, are increased in multiple human blood compartments following ingestion of beetroot juice | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2021-10-27T14:34:03Z | |
dc.identifier.issn | 2213-2317 | |
dc.description | This is the final version. Available from Elsevier via the DOI in this record. | en_GB |
dc.identifier.journal | Redox Biology | en_GB |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2021-04-10 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2021-04-18 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2021-10-27T14:30:52Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2021-10-27T14:34:17Z | |
refterms.panel | C | en_GB |
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Except where otherwise noted, this item's licence is described as © 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)