GnRH pulse generator frequency is modulated by kisspeptin and GABA‐glutamate interactions in the posterodorsal medial amygdala in female mice
dc.contributor.author | Lass, G | |
dc.contributor.author | Li, XF | |
dc.contributor.author | Voliotis, M | |
dc.contributor.author | Wall, E | |
dc.contributor.author | de Burgh, RA | |
dc.contributor.author | Ivanova, D | |
dc.contributor.author | McIntyre, C | |
dc.contributor.author | Lin, XH | |
dc.contributor.author | Colledge, WH | |
dc.contributor.author | Lightman, SL | |
dc.contributor.author | Tsaneva‐Atanasova, K | |
dc.contributor.author | O’Byrne, KT | |
dc.date.accessioned | 2022-10-24T08:09:25Z | |
dc.date.issued | 2022-10-17 | |
dc.date.updated | 2022-10-22T13:44:20Z | |
dc.description.abstract | Kisspeptin neurons in the arcuate nucleus of the hypothalamus generate GnRH pulses, and act as critical initiators of functional gonadotrophin secretion, and reproductive competency. However, kisspeptin in other brain regions, most notably the posterodorsal subnucleus of the medial amygdala (MePD), plays a significant modulatory role over the hypothalamic kisspeptin population; our recent studies using optogenetics have shown that low frequency light stimulation of MePD kisspeptin results in increased LH pulse frequency. Nonetheless, the neurochemical pathways that underpin this regulatory function remain unknown. To study this, we have utilised an optofluid technology, precisely combining optogenetic stimulation with intra-nuclear pharmacological receptor antagonism, to investigate the neurotransmission involved in this circuitry. We have shown experimentally and verified using a mathematical model that functional neurotransmission of both GABA and glutamate is a requirement for effective modulation of the GnRH pulse generator by amygdala kisspeptin neurons. | en_GB |
dc.description.sponsorship | Medical Research Council (MRC) | en_GB |
dc.description.sponsorship | Biotechnology and Biological Sciences Research Council (BBSRC) | en_GB |
dc.description.sponsorship | Engineering and Physical Sciences Research Council (EPSRC) | en_GB |
dc.description.sponsorship | Ford Physiology Fund | en_GB |
dc.identifier.citation | Article e13207 | en_GB |
dc.identifier.doi | https://doi.org/10.1111/jne.13207 | |
dc.identifier.grantnumber | MR/N022637/1 | en_GB |
dc.identifier.grantnumber | BB/S000550/1 | en_GB |
dc.identifier.grantnumber | EP/T017856/1 | en_GB |
dc.identifier.grantnumber | BB/S001255/1 | en_GB |
dc.identifier.grantnumber | BB/S019979/1 | en_GB |
dc.identifier.grantnumber | BB/W005883/1 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/131388 | |
dc.identifier | ORCID: 0000-0001-6488-7198 (Voliotis, Margaritis) | |
dc.language.iso | en | en_GB |
dc.publisher | Wiley | en_GB |
dc.rights | © 2022 The Authors. Journal of Neuroendocrinology published by John Wiley & Sons Ltd on behalf of British Society for Neuroendocrinology. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | en_GB |
dc.subject | amygdala | en_GB |
dc.subject | kisspeptin | en_GB |
dc.subject | LH | en_GB |
dc.subject | GABA | en_GB |
dc.subject | glutamate | en_GB |
dc.title | GnRH pulse generator frequency is modulated by kisspeptin and GABA‐glutamate interactions in the posterodorsal medial amygdala in female mice | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2022-10-24T08:09:25Z | |
dc.identifier.issn | 0953-8194 | |
dc.description | This is the final version. Available on open access from Wiley via the DOI in this record | en_GB |
dc.description | Data Availability Statement: All data contained within the manuscript have been deposited in the King’s Research Data Management System and are freely available to public access (www.kcl.ac.uk/library/researchsupport/research-data-management/preserve/deposit-yourdata-with-kings3). | en_GB |
dc.identifier.eissn | 1365-2826 | |
dc.identifier.journal | Journal of Neuroendocrinology | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2022-10-17 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2022-10-24T08:05:56Z | |
refterms.versionFCD | AM | |
refterms.dateFOA | 2022-10-24T08:09:31Z | |
refterms.panel | B | en_GB |
refterms.dateFirstOnline | 2022-10-17 |
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Except where otherwise noted, this item's licence is described as © 2022 The Authors. Journal of Neuroendocrinology published by John Wiley & Sons Ltd on behalf of British Society for Neuroendocrinology. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium,
provided the original work is properly cited.