Phenotyping single-cell motility in microfluidic confinement.
dc.contributor.author | Bentley, SA | |
dc.contributor.author | Laeverenz-Schlogelhofer, H | |
dc.contributor.author | Anagnostidis, V | |
dc.contributor.author | Cammann, J | |
dc.contributor.author | Mazza, MG | |
dc.contributor.author | Gielen, F | |
dc.contributor.author | Wan, KY | |
dc.date.accessioned | 2023-02-01T11:12:54Z | |
dc.date.issued | 2022-11-23 | |
dc.date.updated | 2023-02-01T11:02:03Z | |
dc.description.abstract | The movement trajectories of organisms serve as dynamic read-outs of their behaviour and physiology. For microorganisms this can be difficult to resolve due to their small size and fast movement. Here, we devise a novel droplet microfluidics assay to encapsulate single micron-sized algae inside closed arenas, enabling ultralong high-speed tracking of the same cell. Comparing two model species - Chlamydomonas reinhardtii (freshwater, 2 cilia), and Pyramimonas octopus (marine, 8 cilia), we detail their highly-stereotyped yet contrasting swimming behaviours and environmental interactions. By measuring the rates and probabilities with which cells transition between a trio of motility states (smooth-forward swimming, quiescence, tumbling or excitable backward swimming), we reconstruct the control network that underlies this gait switching dynamics. A simplified model of cell-roaming in circular confinement reproduces the observed long-term behaviours and spatial fluxes, including novel boundary circulation behaviour. Finally, we establish an assay in which pairs of droplets are fused on demand, one containing a trapped cell with another containing a chemical that perturbs cellular excitability, to reveal how aneural microorganisms adapt their locomotor patterns in real-time. | en_GB |
dc.description.sponsorship | European Commission | en_GB |
dc.description.sponsorship | Academy of Medical Sciences | en_GB |
dc.description.sponsorship | Biotechnology and Biological Sciences Research Council | en_GB |
dc.format.extent | e76519- | |
dc.format.medium | Electronic | |
dc.identifier.citation | Vol. 11, article e76519 | en_GB |
dc.identifier.doi | https://doi.org/10.7554/eLife.76519 | |
dc.identifier.grantnumber | 853560 | en_GB |
dc.identifier.grantnumber | SBF003\1160 | en_GB |
dc.identifier.grantnumber | BB/T011777/1 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/132387 | |
dc.identifier | ORCID: 0000-0003-0604-7224 (Gielen, Fabrice) | |
dc.identifier | ORCID: 0000-0002-0291-328X (Wan, Kirsty Y) | |
dc.language.iso | en | en_GB |
dc.publisher | eLife Sciences Publications | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/36416411 | en_GB |
dc.relation.url | https://doi.org/10.5281/zenodo.7226288 | en_GB |
dc.rights | Copyright Bentley, LaeverenzSchlogelhofer et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. | en_GB |
dc.subject | algae | en_GB |
dc.subject | chlamydomonas reinhardtii | en_GB |
dc.subject | cilia | en_GB |
dc.subject | microfluidics | en_GB |
dc.subject | microswimmer | en_GB |
dc.subject | motility | en_GB |
dc.subject | physics of living systems | en_GB |
dc.subject | single cell | en_GB |
dc.title | Phenotyping single-cell motility in microfluidic confinement. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2023-02-01T11:12:54Z | |
dc.identifier.issn | 2050-084X | |
exeter.place-of-publication | England | |
dc.description | This is the final version. Available from eLife Sciences Publications via the DOI in this record. | en_GB |
dc.description | Data availability: New data and analysis codes generated as part of this study are available for download from Zenodo. The dataset includes all raw cell trajectories and motility states, as well as analysis and simulation codes. The following data sets were generated: Bentley SALaeverenz-Schlogelhofer HAnagnostidis VCammann JMazza MGGielen FWan KY (2022) Zenodo Dataset for: Phenotyping single-cell motility in microfluidic confinement. https://doi.org/10.5281/zenodo.7226288 | en_GB |
dc.identifier.journal | eLife | en_GB |
dc.relation.ispartof | Elife, 11 | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2022-10-13 | |
dc.rights.license | CC BY | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2022-11-23 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2023-02-01T11:09:32Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2023-02-01T11:12:55Z | |
refterms.panel | B | en_GB |
refterms.dateFirstOnline | 2022-11-23 |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's licence is described as Copyright Bentley, LaeverenzSchlogelhofer et al. This article
is distributed under the terms
of the Creative Commons
Attribution License, which
permits unrestricted use and
redistribution provided that the
original author and source are
credited.