Show simple item record

dc.contributor.authorFerrario, A
dc.contributor.authorSaccomanno, V
dc.contributor.authorZhang, H-Y
dc.contributor.authorBorisyuk, R
dc.contributor.authorLi, W-C
dc.date.accessioned2023-02-01T13:03:00Z
dc.date.issued2023-01-24
dc.date.updated2023-02-01T11:59:31Z
dc.description.abstractDeveloping spinal circuits generate patterned motor outputs while many neurons with high membrane resistances are still maturing. In the spinal cord of hatchling frog tadpoles of unknown sex, we found that the firing reliability in swimming of inhibitory interneurons with commissural and ipsilateral ascending axons was negatively correlated with their cellular membrane resistance. Further analyses showed that neurons with higher resistances had outward rectifying properties, low firing thresholds and little delay in firing evoked by current injections. Input synaptic currents these neurons received during swimming, either compound unitary current amplitudes or unitary synaptic current numbers, were scaled with their membrane resistances, but their own synaptic outputs were correlated with membrane resistances of their postsynaptic partners. Analyses of neuronal dendritic and axonal lengths and their activities in swimming and cellular input resistances did not reveal a clear correlation pattern. Incorporating these electrical and synaptic properties in a computer swimming model produced robust swimming rhythms whereas randomising input synaptic strengths led to the breakdown of swimming rhythms, coupled with less synchronised spiking in the inhibitory interneurons. We conclude that the recruitment of these developing interneurons in swimming can be predicted by cellular input resistances, but the order is opposite to the motor-strength based recruitment scheme depicted by Henneman's size principle. This form of recruitment/integration order in development before the emergence of refined motor control is progressive potentially with neuronal acquisition of mature electrical and synaptic properties, among which the scaling of input synaptic strengths with cellular input resistance plays a critical role.SIGNIFICANCE STATEMENT:The mechanisms on how interneurons are recruited to participate circuit function in developing neuronal systems are rarely investigated. In two days old frog tadpole spinal cord, we found the recruitment of inhibitory interneurons in swimming is inversely correlated with cellular input resistances, opposite to the motor-strength based recruitment order depicted by Henneman's size principle. Further analyses showed the amplitude of synaptic inputs neurons received during swimming was inversely correlated with cellular input resistances. Randomising/reversing the relation between input synaptic strengths and membrane resistances in modelling broke down swimming rhythms. Therefore, the recruitment or integration of these interneurons is conditional upon the acquisition of several electrical and synaptic properties including the scaling of input synaptic strengths with cellular input resistances.en_GB
dc.description.sponsorshipBiotechnology & Biological Sciences Research Council (BBSRC)en_GB
dc.description.sponsorshipBiotechnology & Biological Sciences Research Council (BBSRC)en_GB
dc.format.extentjn-rm-0520-22-
dc.format.mediumPrint-Electronic
dc.identifier.citationPublished online 23 January 2023en_GB
dc.identifier.doihttps://doi.org/10.1523/JNEUROSCI.0520-22.2022
dc.identifier.grantnumberBB/L000814/1en_GB
dc.identifier.grantnumberBB/T003146en_GB
dc.identifier.urihttp://hdl.handle.net/10871/132388
dc.identifierORCID: 0000-0003-1384-9057 (Borisyuk, Roman)
dc.identifierScopusID: 7004056663 (Borisyuk, Roman)
dc.identifierResearcherID: A-7476-2014 (Borisyuk, Roman)
dc.language.isoenen_GB
dc.publisherSociety for Neuroscienceen_GB
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pubmed/36693757en_GB
dc.rights.embargoreasonUnder embargo until 24 June 2023 in compliance with publisher policyen_GB
dc.rights© 2023 the authors.en_GB
dc.titleMechanisms underlying the recruitment of inhibitory interneurons in fictive swimming in developing Xenopus laevis tadpoles.en_GB
dc.typeArticleen_GB
dc.date.available2023-02-01T13:03:00Z
dc.identifier.issn0270-6474
exeter.place-of-publicationUnited States
dc.descriptionThis is the author accepted manuscript. The final version is available from the Society for Neuroscience via the DOI in this record en_GB
dc.identifier.eissn1529-2401
dc.identifier.journalThe Journal of Neuroscienceen_GB
dc.relation.ispartofJ Neurosci
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_GB
dcterms.dateAccepted2022-12-02
rioxxterms.versionAMen_GB
rioxxterms.licenseref.startdate2023-01-24
rioxxterms.typeJournal Article/Reviewen_GB
refterms.dateFCD2023-02-01T12:57:13Z
refterms.versionFCDAM
refterms.panelBen_GB
refterms.dateFirstOnline2023-01-24


Files in this item

This item appears in the following Collection(s)

Show simple item record

© 2023 the authors.
Except where otherwise noted, this item's licence is described as © 2023 the authors.