Human myeloid progenitor glucocorticoid receptor activation causes genomic instability, type 1 IFN- response pathway activation and senescence in differentiated microglia; an early life stress model
dc.contributor.author | Wei, J | |
dc.contributor.author | Arber, C | |
dc.contributor.author | Wray, S | |
dc.contributor.author | Hardy, J | |
dc.contributor.author | Piers, TM | |
dc.contributor.author | Pocock, JM | |
dc.date.accessioned | 2023-10-16T10:34:10Z | |
dc.date.issued | 2022-12-26 | |
dc.date.updated | 2023-10-16T09:48:03Z | |
dc.description.abstract | One form of early life stress, prenatal exposure to glucocorticoids (GCs), confers a higher risk of psychiatric and neurodevelopmental disorders in later life. Increasingly, the importance of microglia in these disorders is recognized. Studies on GCs exposure during microglial development have been limited, and there are few, if any, human studies. We established an in vitro model of ELS by continuous pre-exposure of human iPS-microglia to GCs during primitive hematopoiesis (the critical stage of iPS-microglial differentiation) and then examined how this exposure affected the microglial phenotype as they differentiated and matured to microglia, using RNA-seq analyses and functional assays. The iPS-microglia predominantly expressed glucocorticoid receptors over mineralocorticoid receptors, and in particular, the GR-α splice variant. Chronic GCs exposure during primitive hematopoiesis was able to recapitulate in vivo ELS effects. Thus, pre-exposure to prolonged GCs resulted in increased type I interferon signaling, the presence of Cyclic GMP-AMP synthase-positive (cGAS) micronuclei, cellular senescence and reduced proliferation in the matured iPS-microglia. The findings from this in vitro ELS model have ramifications for the responses of microglia in the pathogenesis of GC- mediated ELS-associated disorders such as schizophrenia, attention-deficit hyperactivity disorder and autism spectrum disorder. | en_GB |
dc.description.sponsorship | Alzheimer’s Society | en_GB |
dc.description.sponsorship | Alzheimer's Research UK | en_GB |
dc.description.sponsorship | National Institute for Health and Care Research (NIHR) | en_GB |
dc.format.extent | 1036-1056 | |
dc.format.medium | Print-Electronic | |
dc.identifier.citation | Vol. 71(4), pp. 1036-1056 | en_GB |
dc.identifier.doi | https://doi.org/10.1002/glia.24325 | |
dc.identifier.grantnumber | AS-JF-18-008 | en_GB |
dc.identifier.grantnumber | ARUK- SRF2016B-2 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/134265 | |
dc.identifier | ORCID: 0000-0003-1015-3417 (Piers, Thomas M) | |
dc.language.iso | en | en_GB |
dc.publisher | Wiley | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/36571248 | en_GB |
dc.rights | © 2022 The Authors. GLIA published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | en_GB |
dc.subject | cellular senescence | en_GB |
dc.subject | early life stress | en_GB |
dc.subject | genomic instability | en_GB |
dc.subject | glucocorticoid receptor | en_GB |
dc.subject | glucocorticoids | en_GB |
dc.subject | induced pluripotent stem cells | en_GB |
dc.subject | microglia | en_GB |
dc.subject | micronuclei | en_GB |
dc.title | Human myeloid progenitor glucocorticoid receptor activation causes genomic instability, type 1 IFN- response pathway activation and senescence in differentiated microglia; an early life stress model | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2023-10-16T10:34:10Z | |
dc.identifier.issn | 0894-1491 | |
exeter.place-of-publication | United States | |
dc.description | This is the final version. Available on open access from Wiley via the DOI in this record | en_GB |
dc.description | Data availability statement: Accession codes: Gene Expression Omnibus GSE73721 (Zhang et al., 2016), GSE99074 (Galatro et al., 2017), dbGaP: phs001373.v2.p2 (Gosselin et al., 2017), SYNAPSE: syn3219045, syn11468526 (Olah et al., 2018).Our RNA-Seq data is available on request. | en_GB |
dc.identifier.eissn | 1098-1136 | |
dc.identifier.journal | Glia | en_GB |
dc.relation.ispartof | Glia, 71(4) | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | en_GB |
dcterms.dateAccepted | 2022-12-09 | |
dc.rights.license | CC BY-NC-ND | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2022-12-26 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2023-10-16T10:31:30Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2023-10-16T10:34:11Z | |
refterms.panel | A | en_GB |
refterms.dateFirstOnline | 2022-12-26 |
Files in this item
This item appears in the following Collection(s)
Except where otherwise noted, this item's licence is described as © 2022 The Authors. GLIA published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any
medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.