Naturally derived phenethyl isothiocyanate modulates induction of oxidative stress via Its N-acetylated cysteine conjugated form in malignant melanoma
dc.contributor.author | Kyriakou, S | |
dc.contributor.author | Demosthenous, N | |
dc.contributor.author | Amery, T | |
dc.contributor.author | Stewart, KJ | |
dc.contributor.author | Winyard, PG | |
dc.contributor.author | Franco, R | |
dc.contributor.author | Pappa, A | |
dc.contributor.author | Panayiotidis, MI | |
dc.date.accessioned | 2024-04-05T15:31:44Z | |
dc.date.issued | 2024-01-08 | |
dc.date.updated | 2024-04-05T14:58:15Z | |
dc.description.abstract | Phenethyl isothiocyanate (PEITC) is a secondary metabolic product yielded upon the hydrolysis of gluconasturtiin and it is highly accumulated in the flowers of watercress. The aim of the current study was to assess the role of a naturally derived PEITC-enriched extract in the induction of oxidative stress and to evaluate its anti-melanoma potency through the regulation of its metabolism with the concurrent production of the N-acetyl cysteine conjugated by-product. For this purpose, an in vitro melanoma model was utilized consisting of human primary (A375) cells as well as metastatic (COLO-679) malignant melanoma cells together with non-tumorigenic immortalized keratinocytes (HaCaT). Cytotoxicity was assessed via the Alamar Blue assay whereas the antioxidant/prooxidant activity of PEITC was determined via spectrophotometric assays. Finally, kinetic characterization of the end-product of PEITC metabolism was monitored via UPLC coupled to a tandem mass spectrometry (MS/MS). Our results indicate that although PhEF showed very minor antioxidant activity in a cell-free system, in a cell-based system, it can modulate the activity of key enzyme(s) involved in cellular antioxidant defense mechanism(s). In addition, we have shown that PhEF induces lipid and protein oxidation in a concentration-dependent manner, while its cytotoxicity is not only dependent on PEITC itself but also on its N-acetylated cysteine conjugated form. | en_GB |
dc.description.sponsorship | Hellenic Foundation for Research and Innovation (H.F.R.I.) | en_GB |
dc.description.sponsorship | Cyprus Institute of Neurology and Genetics (Telethon Cyprus) | en_GB |
dc.identifier.citation | Vol. 13, article 82 | en_GB |
dc.identifier.doi | https://doi.org/10.3390/antiox13010082 | |
dc.identifier.grantnumber | HFRI-FM17C3- 2007 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/135696 | |
dc.identifier | ORCID: 0000-0002-9613-1202 (Winyard, Paul G) | |
dc.language.iso | en | en_GB |
dc.publisher | MDPI | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/38247506 | en_GB |
dc.rights | Copyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/) | en_GB |
dc.subject | antioxidant enzymes | en_GB |
dc.subject | isothiocyanates | en_GB |
dc.subject | malignant melanoma | en_GB |
dc.subject | mercapturic acid pathway | en_GB |
dc.subject | oxidative stress | en_GB |
dc.subject | phenethyl isothiocyanate | en_GB |
dc.subject | polyphenols | en_GB |
dc.subject | watercress | en_GB |
dc.title | Naturally derived phenethyl isothiocyanate modulates induction of oxidative stress via Its N-acetylated cysteine conjugated form in malignant melanoma | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2024-04-05T15:31:44Z | |
dc.identifier.issn | 2076-3921 | |
exeter.article-number | 82 | |
exeter.place-of-publication | Switzerland | |
dc.description | This is the final version. Available on open access from MDPI via the DOI in this record. | en_GB |
dc.description | Data Availability Statement: Data are contained within the article. | en_GB |
dc.identifier.journal | Antioxidants | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2024-01-05 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2024-01-08 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2024-04-05T15:28:52Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2024-04-05T15:31:55Z | |
refterms.panel | A | en_GB |
refterms.dateFirstOnline | 2024-01-08 |
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