Single Molecule Thermodynamic Penalties Applied to Enzymes by Whispering Gallery Mode Biosensors
dc.contributor.author | Houghton, MC | |
dc.contributor.author | Toropov, NA | |
dc.contributor.author | Yu, D | |
dc.contributor.author | Bagby, S | |
dc.contributor.author | Vollmer, F | |
dc.date.accessioned | 2024-07-22T12:15:16Z | |
dc.date.issued | 2024-07-12 | |
dc.date.updated | 2024-07-18T16:34:36Z | |
dc.description.abstract | Optical microcavities, particularly whispering gallery mode (WGM) microcavities enhanced by plasmonic nanorods, are emerging as powerful platforms for single-molecule sensing. However, the impact of optical forces from the plasmonic near field on analyte molecules is inadequately understood. Using a standard optoplasmonic WGM single-molecule sensor to monitor two enzymes, both of which undergo an open-to-closed-to-open conformational transition, the work done on an enzyme by the WGM sensor as atoms of the enzyme move through the electric field gradient of the plasmonic hotspot during conformational change has been quantified. As the work done by the sensor on analyte enzymes can be modulated by varying WGM intensity, the WGM microcavity system can be used to apply free energy penalties to regulate enzyme activity at the single-molecule level. The findings advance the understanding of optical forces in WGM single-molecule sensing, potentially leading to the capability to precisely manipulate enzyme activity at the single-molecule level through tailored optical modulation. | en_GB |
dc.description.sponsorship | Biotechnology and Biological Sciences Research Council (BBSRC) | en_GB |
dc.description.sponsorship | Engineering and Physical Sciences Research Council (EPSRC) | en_GB |
dc.format.extent | e2403195- | |
dc.format.medium | Print-Electronic | |
dc.identifier.citation | Article e2403195 | en_GB |
dc.identifier.doi | https://doi.org/10.1002/advs.202403195 | |
dc.identifier.grantnumber | BB/T008741/1 | en_GB |
dc.identifier.grantnumber | BB/Y512977/1 | en_GB |
dc.identifier.grantnumber | EP/T002875/1 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/136811 | |
dc.identifier | ORCID: 0000-0001-7570-6348 (Houghton, Matthew C) | |
dc.language.iso | en | en_GB |
dc.publisher | Wiley | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/38995192 | en_GB |
dc.rights | © 2024 The Author(s). Advanced Science published by Wiley-VCH GmbH. . Advanced Science published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | en_GB |
dc.subject | biosensors | en_GB |
dc.subject | enzymes | en_GB |
dc.subject | optical forces | en_GB |
dc.subject | optoplasmonics | en_GB |
dc.subject | peptides and proteins | en_GB |
dc.subject | plasmonics | en_GB |
dc.title | Single Molecule Thermodynamic Penalties Applied to Enzymes by Whispering Gallery Mode Biosensors | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2024-07-22T12:15:16Z | |
dc.identifier.issn | 2198-3844 | |
exeter.place-of-publication | Germany | |
dc.description | This is the final version. Available on open access from Wiley via the DOI in this record | en_GB |
dc.description | Data Availability Statement: The data that support the findings of this study are available in the supplementary material of this article. | en_GB |
dc.identifier.eissn | 2198-3844 | |
dc.identifier.journal | Advanced Science | en_GB |
dc.relation.ispartof | Adv Sci (Weinh) | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2024-07-01 | |
dcterms.dateSubmitted | 2024-03-26 | |
dc.rights.license | CC BY | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2024-07-12 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2024-07-18T16:34:38Z | |
refterms.versionFCD | AM | |
refterms.dateFOA | 2024-07-22T12:15:47Z | |
refterms.panel | B | en_GB |
refterms.dateFirstOnline | 2024-07-12 | |
exeter.rights-retention-statement | Yes |
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Except where otherwise noted, this item's licence is described as © 2024 The Author(s). Advanced Science published by Wiley-VCH GmbH. . Advanced Science published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.