Network balance via CRY signalling controls the Arabidopsis circadian clock over ambient temperatures
Millar, Andrew J.
Molecular Systems Biology
Nature Publishing Group: Open Access Journals - Option B
Molecular Systems Biology is an open-access journal published by the European Molecular BiologyOrganizationandNaturePublishingGroup.Thiswork is licensed under a Creative Commons Attribution-Noncommercial- Share Alike 3.0 Unported Licence. To view a copy of this licence visit http://creativecommons.org/licenses/bync- sa/3.0/.
Circadian clocks exhibit 'temperature compensation', meaning that they show only small changes in period over a broad temperature range. Several clock genes have been implicated in the temperature-dependent control of period in Arabidopsis. We show that blue light is essential for this, suggesting that the effects of light and temperature interact or converge upon common targets in the circadian clock. Our data demonstrate that two cryptochrome photoreceptors differentially control circadian period and sustain rhythmicity across the physiological temperature range. In order to test the hypothesis that the targets of light regulation are sufficient to mediate temperature compensation, we constructed a temperature-compensated clock model by adding passive temperature effects into only the light-sensitive processes in the model. Remarkably, this model was not only capable of full temperature compensation and consistent with mRNA profiles across a temperature range, but also predicted the temperature-dependent change in the level of LATE ELONGATED HYPOCOTYL, a key clock protein. Our analysis provides a systems-level understanding of period control in the plant circadian oscillator.
BBSRC/EPSRC‐funded ROBuST SABR project (BB/F005237/1, BB/F005318/1, BB/F005261/1 and BB/F005296/1) led by Karen Halliday.
notes: PMCID: PMC3619941
types: Journal Article; Research Support, Non-U.S. Gov't
Vol. 9, pp. 650 -
Place of publication