NADPH oxidases regulate septin-mediated cytoskeletal remodeling during plant infection by the rice blast fungus
Dagdas, Yasin Fatih
Talbot, Nicholas J.
Proc Natl Acad Sci U S A
Please cite published version which is available from the DOI link above.
The rice blast fungus Magnaporthe oryzae infects plants with a specialized cell called an appressorium, which uses turgor to drive a rigid penetration peg through the rice leaf cuticle. Here, we show that NADPH oxidases (Nox) are necessary for septin-mediated reorientation of the F-actin cytoskeleton to facilitate cuticle rupture and plant cell invasion. We report that the Nox2-NoxR complex spatially organizes a heteroligomeric septin ring at the appressorium pore, required for assembly of a toroidal F-actin network at the point of penetration peg emergence. Maintenance of the cortical F-actin network during plant infection independently requires Nox1, a second NADPH oxidase, which is necessary for penetration hypha elongation. Organization of F-actin in appressoria is disrupted by application of antioxidants, whereas latrunculin-mediated depolymerization of appressorial F-actin is competitively inhibited by reactive oxygen species, providing evidence that regulated synthesis of reactive oxygen species by fungal NADPH oxidases directly controls septin and F-actin dynamics.
Biotechnology and Biological Sciences Research Council (BBSRC)
National Natural Science Foundation of China
European Research Council Advanced Investigator Award
notes: PMCID: PMC3581893
types: Journal Article; Research Support, Non-U.S. Gov't
Vol. 110, Issue 8, pp. 3179 - 3184
Place of publication