dc.contributor.author | Olkhov, RV | |
dc.contributor.author | Kaminski, ER | |
dc.contributor.author | Shaw, AM | |
dc.date.accessioned | 2015-04-23T08:59:27Z | |
dc.date.issued | 2012-11 | |
dc.description.abstract | A label-free nanoparticle array platform has been used to detect total peanut allergen-specific binding from whole serum of patients suffering from peanut allergy. The serum from 10 patients was screened against a four-allergen panel of cat and dog dander, dust mite and peanut allergen protein Ara h1. The IgE and IgG contributions to the total specific-binding protein load to Ara h1 were identified using two secondary IgG- and IgE-specific antibodies and were found to contribute less than 50 % of the total specific protein load. The total mass of IgE, IgE and the unresolved specific-binding protein ΔsBP for Ara h1 provides a new serum profile for high-RAST-grade patients 5 and 6 with the IgG/IgE ratio of 4 ± 2 and ΔsBP/IgE ratio of 17 ± 11, neither of which is protective for the small patient cohort. | en_GB |
dc.description.sponsorship | BBSRC | en_GB |
dc.description.sponsorship | EPSRC | en_GB |
dc.identifier.citation | Vol. 404, Iss. 8, pp. 2241 - 2247 | en_GB |
dc.identifier.doi | 10.1007/s00216-012-6390-z | |
dc.identifier.uri | http://hdl.handle.net/10871/16967 | |
dc.language.iso | en | en_GB |
dc.publisher | Springer Verlag (Germany) | en_GB |
dc.relation.url | http://www.ncbi.nlm.nih.gov/pubmed/22983170 | en_GB |
dc.relation.url | http://link.springer.com/article/10.1007%2Fs00216-012-6390-z | en_GB |
dc.rights | The final publication is available at Springer via http://dx.doi.org/ 10.1007/s00216-012-6390-z | en_GB |
dc.subject | Allergens | en_GB |
dc.subject | Animals | en_GB |
dc.subject | Cats | en_GB |
dc.subject | Dogs | en_GB |
dc.subject | Gold | en_GB |
dc.subject | Humans | en_GB |
dc.subject | Immunoassay | en_GB |
dc.subject | Kinetics | en_GB |
dc.subject | Mice | en_GB |
dc.subject | Nanoparticles | en_GB |
dc.subject | Peanut Hypersensitivity | en_GB |
dc.subject | Protein Binding | en_GB |
dc.subject | Surface Plasmon Resonance | en_GB |
dc.title | Differential immuno-kinetic assays of allergen-specific binding for peanut allergy serum analysis. | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2015-04-23T08:59:27Z | |
dc.identifier.issn | 1618-2642 | |
exeter.place-of-publication | Germany | |
dc.description | Journal Article | en_GB |
dc.description | Research Support, Non-U.S. Gov't | en_GB |
dc.description | Copyright © Springer-Verlag 2012 | en_GB |
dc.identifier.journal | Analytical and Bioanalytical Chemistry | en_GB |