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dc.contributor.authorMansfield, Jessica
dc.contributor.authorMoger, Julian
dc.contributor.authorGreen, Ellen
dc.contributor.authorMoger, C
dc.contributor.authorWinlove, C. Peter
dc.date.accessioned2015-11-09T12:03:43Z
dc.date.issued2013-01-10
dc.description.abstractStimulated Raman scattering (SRS) has been applied to unstained samples of articular cartilage enabling the investigation of living cells within fresh tissue. Hyperspectral SRS measurements over the CH vibrational region showed variations in protein and lipid content within the cells, pericellular matrix and interterritorial matrix. Changes in the cells and pericellular matrix were investigated as a function of depth into the cartilage. Lipid was detected in the pericellular matrix of superficial zone chondrocytes. The spectral profile of lipid droplets within the chondrocytes indicated that they contained predominantly unsaturated lipids. The mineral content has been imaged by using the PO₄³⁻ vibration at 959 cm⁻¹ and the CO₃²⁻ vibration at 1070 cm⁻¹. Both changes in cells and mineralization are known to be important factors in the progression of osteoarthritis. SRS enables these to be visualized in fresh unstained tissue and consequently should benefit osteoarthiritis research.en_GB
dc.identifier.citationVol. 6, Issue 10, pp. 803 - 814, October 2013en_GB
dc.identifier.doi10.1002/jbio.201200213
dc.identifier.urihttp://hdl.handle.net/10871/18599
dc.language.isoenen_GB
dc.publisherWiley-VCH Verlagen_GB
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/23303610en_GB
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1002/jbio.201200213/abstracten_GB
dc.subjectcartilageen_GB
dc.subjectcoherent anti-Stokes Raman scatteringen_GB
dc.subjectmineralized tissueen_GB
dc.subjectsecond-harmonic generationen_GB
dc.subjectspontaneous Ramanen_GB
dc.subjectstimulated Raman scatteringen_GB
dc.subjecttwo-photon fluorescenceen_GB
dc.subjectAnimalsen_GB
dc.subjectCalcification, Physiologicen_GB
dc.subjectCartilage, Articularen_GB
dc.subjectExtracellular Matrixen_GB
dc.subjectHorsesen_GB
dc.subjectIntracellular Spaceen_GB
dc.subjectMolecular Imagingen_GB
dc.subjectSpectrum Analysis, Ramanen_GB
dc.titleChemically specific imaging and in-situ chemical analysis of articular cartilage with stimulated Raman scatteringen_GB
dc.typeArticleen_GB
dc.date.available2015-11-09T12:03:43Z
dc.identifier.issn1864-063X
exeter.place-of-publicationGermany
dc.descriptionThis is the pre-peer reviewed version of the following article: Mansfield, J., Moger, J., Green, E., Moger, C. and Winlove, C. P. (2013), Chemically specific imaging and in-situ chemical analysis of articular cartilage with stimulated Raman scattering. J. Biophoton., 6: 803–814. doi: 10.1002/jbio.201200213, which has been published in final form at http://dx.doi.org/10.1002/jbio.201200213.en_GB
dc.description© 2013 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheimen_GB
dc.identifier.eissn1864-0648
dc.identifier.journalJournal of Biophotonicsen_GB
dc.identifier.pmid23303610


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