Brillouin spectroscopy applied to protein fibers of the extracellular matrix
Journal of Visualized Experiments
Journal of Visualized Experiments (JoVE)
This is the author accepted manuscript. The final version is available under a Creative Commons Attribution 3.0 License at Journal of Visualized Experiments (JoVE). Paid for Gold.
Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy
Brillouin spectroscopy is an emerging technique in the biomedical field. It probes the mechanical properties of a sample through the interaction of visible light with thermally induced acoustic waves or phonons propagating at a speed of a few km/sec. Information on the elasticity and structure of the material is obtained in a nondestructive contactless manner, hence opening the way to in vivo applications and potential diagnosis of pathology. This work describes the application of Brillouin spectroscopy to the study of biomechanics in elastin and trypsin-digested type I collagen fibers of the extracellular matrix. Fibrous proteins of the extracellular matrix are the building blocks of biological tissues and investigating their mechanical and physical behavior is key to establishing structure–function relationships in normal tissues and the changes which occur in disease. The procedures of sample preparation followed by measurement of Brillouin spectra using a reflective substrate are presented together with details of the optical system and methods of spectral data analysis.
This work was supported by the Engineering and Physical Sciences Research Council [grant number EP/M028739/1]. RSE was supported by a Santander Postgraduate Research Award 2015.
Issue 115, Article no. e54648