dc.contributor.author | Garrett, NL | |
dc.contributor.author | Godfrey, L | |
dc.contributor.author | Lalatsa, A | |
dc.contributor.author | Serrano, DR | |
dc.contributor.author | Uchegbu, IF | |
dc.contributor.author | Schatzlein, A | |
dc.contributor.author | Moger, J | |
dc.date.accessioned | 2016-06-14T11:19:22Z | |
dc.date.issued | 2015-03-05 | |
dc.description.abstract | © 2015 SPIE.Recent advances in pharmaceutical nanotechnology have enabled the development of nano-particulate medicines with enhanced drug performance. Although the fate of these nano-particles can be macroscopically tracked in the body (e.g. using radio-labeling techniques), there is little information about the sub-cellular scale mechanistic processes underlying the particle-tissue interactions, or how these interactions may correlate with pharmaceutical efficacy. To rationally engineer these nano-particles and thus optimize their performance, these mechanistic interactions must be fully understood. Coherent Anti-Stokes Raman scattering (CARS) microscopy provides a label-free means for visualizing biological samples, but can suffer from a strong non-resonant background in samples that are prepared using aldehyde-based fixatives. We demonstrate how formalin fixative affects the detection of polymeric nanoparticles within kidneys following oral administration using CARS microscopy, compared with samples that were snap-frozen. These findings have implications for clinical applications of CARS for probing nanoparticle distribution in tissue biopsies. | en_GB |
dc.identifier.citation | Proceedings of SPIE, 2015, Vol. 9329 | en_GB |
dc.identifier.doi | 10.1117/12.2079365 | |
dc.identifier.uri | http://hdl.handle.net/10871/22083 | |
dc.language.iso | en | en_GB |
dc.publisher | Society of Photo-optical Instrumentation Engineers (SPIE) | en_GB |
dc.rights | This is the final version of the article. Available from SPIE via the DOI in this record. | en_GB |
dc.title | Detecting polymeric nanoparticles with coherent anti-stokes Raman scattering microscopy in tissues exhibiting fixative-induced autofluorescence | en_GB |
dc.type | Conference proceedings | en_GB |
dc.date.available | 2016-06-14T11:19:22Z | |
dc.identifier.isbn | 9781628414196 | |
dc.identifier.issn | 1605-7422 | |
dc.identifier.journal | Proceedings of SPIE | en_GB |