dc.contributor.author | Cizmeci, D | |
dc.contributor.author | Dempster, EL | |
dc.contributor.author | Champion, OL | |
dc.contributor.author | Wagley, S | |
dc.contributor.author | Akman, OE | |
dc.contributor.author | Prior, JL | |
dc.contributor.author | Soyer, OS | |
dc.contributor.author | Mill, J | |
dc.contributor.author | Titball, RW | |
dc.date.accessioned | 2016-11-28T10:31:14Z | |
dc.date.issued | 2016-08-03 | |
dc.description.abstract | The potential for epigenetic changes in host cells following microbial infection has been widely suggested, but few examples have been reported. We assessed genome-wide patterns of DNA methylation in human macrophage-like U937 cells following infection with Burkholderia pseudomallei, an intracellular bacterial pathogen and the causative agent of human melioidosis. Our analyses revealed significant changes in host cell DNA methylation, at multiple CpG sites in the host cell genome, following infection. Infection induced differentially methylated probes (iDMPs) showing the greatest changes in DNA methylation were found to be in the vicinity of genes involved in inflammatory responses, intracellular signalling, apoptosis and pathogen-induced signalling. A comparison of our data with reported methylome changes in cells infected with M. tuberculosis revealed commonality of differentially methylated genes, including genes involved in T cell responses (BCL11B, FOXO1, KIF13B, PAWR, SOX4, SYK), actin cytoskeleton organisation (ACTR3, CDC42BPA, DTNBP1, FERMT2, PRKCZ, RAC1), and cytokine production (FOXP1, IRF8, MR1). Overall our findings show that pathogenic-specific and pathogen-common changes in the methylome occur following infection. | en_GB |
dc.description.sponsorship | This work was supported by the UK Defence Science and Technology Laboratory, award DSTLX-1000060221 to R.W.T., O.S.S. and O.E.A. | en_GB |
dc.identifier.citation | Vol. 6, article. 30861 | en_GB |
dc.identifier.doi | 10.1038/srep30861 | |
dc.identifier.other | srep30861 | |
dc.identifier.uri | http://hdl.handle.net/10871/24605 | |
dc.language.iso | en | en_GB |
dc.publisher | Nature Publishing Group | en_GB |
dc.relation.url | http://www.ncbi.nlm.nih.gov/pubmed/27484700 | en_GB |
dc.rights | This work is licensed under a Creative Commons Attribution 4.0 International License. The images
or other third party material in this article are included in the article’s Creative Commons license,
unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license,
users will need to obtain permission from the license holder to reproduce the material. To view a copy of this
license, visit http://creativecommons.org/licenses/by/4.0/
© The Author(s) 2016 | en_GB |
dc.title | Mapping epigenetic changes to the host cell genome induced by Burkholderia pseudomallei reveals pathogen-specific and pathogen-generic signatures of infection | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2016-11-28T10:31:14Z | |
dc.identifier.issn | 2045-2322 | |
exeter.place-of-publication | England | en_GB |
dc.description | This is the final version of the article. Available from the publisher via the DOI in this record. | en_GB |
dc.identifier.journal | Scientific Reports | en_GB |
dc.identifier.pmcid | PMC4971488 | |
dc.identifier.pmid | 27484700 | |