Real-time deformability cytometry: On-the-fly cell mechanical phenotyping
Otto, O; Rosendahl, P; Mietke, A; et al.Golfier, S; Herold, C; Klaue, D; Girardo, S; Pagliara, S; Ekpenyong, A; Jacobi, A; Wobus, M; Töpfner, N; Keyser, UF; Mansfeld, J; Fischer-Friedrich, E; Guck, J
Date: 1 January 2015
Article
Journal
Nature Methods
Publisher
Nature Publishing Group
Publisher DOI
Abstract
We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and ...
We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.
Biosciences - old structure
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