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Real-time deformability cytometry: On-the-fly cell mechanical phenotyping

dc.contributor.authorOtto, O
dc.contributor.authorRosendahl, P
dc.contributor.authorMietke, A
dc.contributor.authorGolfier, S
dc.contributor.authorHerold, C
dc.contributor.authorKlaue, D
dc.contributor.authorGirardo, S
dc.contributor.authorPagliara, S
dc.contributor.authorEkpenyong, A
dc.contributor.authorJacobi, A
dc.contributor.authorWobus, M
dc.contributor.authorTöpfner, N
dc.contributor.authorKeyser, UF
dc.contributor.authorMansfeld, J
dc.contributor.authorFischer-Friedrich, E
dc.contributor.authorGuck, J
dc.date.accessioned2017-02-13T13:28:10Z
dc.date.issued2015-01-01
dc.description.abstractWe introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.en_GB
dc.description.sponsorshipWe thank A. Taubenberger, M. Herbig, C. Liebers, L. Menschner, C. Klug, R. Berner, M. Bornhäuser, C. Bryant, E. Chilvers, B. Friedrich, A. Voigt, J. Tegenfeldt, M. Tschöp, F. Amblard, T. Hyman and S. Grill for technical support, advice and engaging discussions. The HL60/S4 cells were a generous gift of D. and A. Olins (University of New England). Financial support from the Alexander-von-Humboldt Stiftung (Humboldt-Professorship to J.G.), Sächsisches Ministerium für Wissenschaft und Kunst (TG70 grant to O.O. and J.G.), DFG-Center for Regenerative Medicine of the Technische Universität Dresden (seed grant to J.G.), Deutsche Forschungsgemeinschaft (DFG Emmy Noether Grant to J.M., MA 5831/1-1; KFO249 Gerok position to N.T.), Deutsche Gesellschaft für Pädiatrische Infektiologie (N.T.) and Studienstiftung des Deutschen Volkes (A.M.), Max Planck Society (E.F.-F.) and Leverhulme and Newton Trust (Early Career Fellowship to S.P.) is gratefully acknowledged.en_GB
dc.identifier.citationVol. 12, pp. 199 - 202en_GB
dc.identifier.doi10.1038/nmeth.3281
dc.identifier.urihttp://hdl.handle.net/10871/25777
dc.language.isoenen_GB
dc.publisherNature Publishing Groupen_GB
dc.subjectBiophysical methodsen_GB
dc.subjectBiotechnologyen_GB
dc.subjectFlow cytometryen_GB
dc.subjectLab-on-a-chipen_GB
dc.titleReal-time deformability cytometry: On-the-fly cell mechanical phenotypingen_GB
dc.typeArticleen_GB
dc.date.available2017-02-13T13:28:10Z
dc.identifier.issn1548-7091
dc.descriptionThis is the author accepted manuscript. The final version is available from the publisher via the DOI in this record.en_GB
dc.identifier.journalNature Methodsen_GB


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