Toward multi-focal spot remote focusing two-photon microscopy for high speed imaging
Proceedings of SPIE
Society of Photo-optical Instrumentation Engineers
Optical sectioning techniques using two-photon excitation of fluorescent indicators are central to diverse imaging applications. The limitations of the technique are low speed and undesirable specimen agitation. In our design, highspeed axial scanning is carried out by moving a reference objective to axially displace the focal spot without introducing significant spherical aberration and any agitation of the specimen. Further, the system is configured to allow switching between single spot and multiple focal spot remote ...
The project is funded by the Medical Research Council through project “MICA: High speed, high resolution imaging of excitable cell networks” (MR/K015877/1).
This is the final version of the article. Available from SPIE via the DOI in this record.
Vol. 10070: Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV, paper 1007005