Burkholderia pseudomallei kynB plays a role in AQ production, biofilm formation, bacterial swarming and persistence
Research in Microbiology
Elsevier for Institut Pasteur
Kynurenine formamidase (KynB) forms part of the kynurenine pathway which metabolises tryptophan to anthranilate. This metabolite can be used for downstream production of 2-alkyl-4-quinolone (AQ) signalling molecules that control virulence in Pseudomonas aeruginosa. Here we investigate the role of kynB in the production of AQs and virulence-associated phenotypes of Burkholderia pseudomallei K96243, the causative agent of melioidosis. Deletion of kynB resulted in reduced AQ production, increased biofilm formation, decreased swarming and increased tolerance to ciprofloxacin. Addition of exogenous anthranilic acid restored the biofilm phenotype, but not the persister phenotype. This study suggests the kynurenine pathway is a critical source of anthranilate and signalling molecules that may regulate B. pseudomallei virulence.
This work was supported by the Defence Science and Technology Laboratory, grant DSTLX-1000060221 (WP2).
This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this record.
Vol. 167 (3), pp. 159 - 167
Place of publication