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dc.contributor.authorButt, A
dc.contributor.authorHalliday, N
dc.contributor.authorWilliams, P
dc.contributor.authorAtkins, HS
dc.contributor.authorBancroft, GJ
dc.contributor.authorTitball, RW
dc.date.accessioned2017-03-16T09:59:41Z
dc.date.issued2015-11-30
dc.description.abstractKynurenine formamidase (KynB) forms part of the kynurenine pathway which metabolises tryptophan to anthranilate. This metabolite can be used for downstream production of 2-alkyl-4-quinolone (AQ) signalling molecules that control virulence in Pseudomonas aeruginosa. Here we investigate the role of kynB in the production of AQs and virulence-associated phenotypes of Burkholderia pseudomallei K96243, the causative agent of melioidosis. Deletion of kynB resulted in reduced AQ production, increased biofilm formation, decreased swarming and increased tolerance to ciprofloxacin. Addition of exogenous anthranilic acid restored the biofilm phenotype, but not the persister phenotype. This study suggests the kynurenine pathway is a critical source of anthranilate and signalling molecules that may regulate B. pseudomallei virulence.en_GB
dc.description.sponsorshipThis work was supported by the Defence Science and Technology Laboratory, grant DSTLX-1000060221 (WP2).en_GB
dc.identifier.citationVol. 167 (3), pp. 159 - 167en_GB
dc.identifier.doi10.1016/j.resmic.2015.11.002
dc.identifier.urihttp://hdl.handle.net/10871/26619
dc.language.isoenen_GB
dc.publisherElsevier for Institut Pasteuren_GB
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pubmed/26654915en_GB
dc.subjectAnthranilateen_GB
dc.subjectAntibiotic toleranceen_GB
dc.subjectKynurenineen_GB
dc.subjectQuorum sensingen_GB
dc.subjectVirulence factorsen_GB
dc.subjectArylformamidaseen_GB
dc.subjectBiofilmsen_GB
dc.subjectBurkholderia pseudomalleien_GB
dc.subjectGene Deletionen_GB
dc.subjectLocomotionen_GB
dc.subjectQuinolonesen_GB
dc.subjectSignal Transductionen_GB
dc.subjectTryptophanen_GB
dc.subjectVirulenceen_GB
dc.subjectortho-Aminobenzoatesen_GB
dc.titleBurkholderia pseudomallei kynB plays a role in AQ production, biofilm formation, bacterial swarming and persistenceen_GB
dc.typeArticleen_GB
dc.date.available2017-03-16T09:59:41Z
exeter.place-of-publicationFranceen_GB
dc.descriptionThis is the author accepted manuscript. The final version is available from Elsevier via the DOI in this record.en_GB
dc.identifier.journalResearch in Microbiologyen_GB


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