dc.contributor.author | McQuillan, JS | |
dc.contributor.author | Shaw, AM | |
dc.date.accessioned | 2017-04-19T15:16:15Z | |
dc.date.issued | 2014-01-06 | |
dc.description.abstract | We report the whole-transcriptome response of Escherichia coli bacteria to acute treatment with silver nanoparticles (AgNPs) or silver ions [Ag(I)] as silver nitrate using gene expression microarrays. In total, 188 genes were regulated by both silver treatments, 161 were up-regulated and 27 were down-regulated. Significant regulation was observed for heat shock response genes in line with protein denaturation associated with protein structure vulnerability indicating Ag(I)-labile -SH bonds. Disruption to iron-sulphur clusters led to the positive regulation of iron-sulphur assembly systems and the expression of genes for iron and sulphate homeostasis. Further, Ag ions induced a redox stress response associated with large (>600-fold) up-regulation of the E. coli soxS transcriptional regulator gene. Ag(I) is isoelectronic with Cu(I), and genes associated with copper homeostasis were positively regulated indicating Ag(I)-activation of copper signalling. Differential gene expression was observed for the silver nitrate and AgNP silver delivery. Nanoparticle delivery of Ag(I) induced the differential regulation of 379 genes; 309 genes were uniquely regulated by silver nanoparticles and 70 genes were uniquely regulated by silver nitrate. The differential silver nanoparticle-silver nitrate response indicates that the toxic effect of labile Ag(I) in the system depends upon the mechanism of delivery to the target cell. | en_GB |
dc.description.sponsorship | JM would like to thank the BBSRC for a CASE studentship award with ENBL ltd. | en_GB |
dc.identifier.citation | Vol. 8 supplement 1, pp. 177 - 184 | en_GB |
dc.identifier.doi | 10.3109/17435390.2013.870243 | |
dc.identifier.uri | http://hdl.handle.net/10871/27160 | |
dc.language.iso | en | en_GB |
dc.publisher | Taylor & Francis | en_GB |
dc.relation.url | https://www.ncbi.nlm.nih.gov/pubmed/24392705 | en_GB |
dc.subject | Mechanism | en_GB |
dc.subject | microarray | en_GB |
dc.subject | nanoparticle | en_GB |
dc.subject | silver | en_GB |
dc.subject | toxicity | en_GB |
dc.subject | Escherichia coli | en_GB |
dc.subject | Gene Expression Regulation | en_GB |
dc.subject | Metal Nanoparticles | en_GB |
dc.subject | Silver | en_GB |
dc.subject | Transcriptome | en_GB |
dc.title | Differential gene regulation in the Ag nanoparticle and Ag(+)-induced silver stress response in Escherichia coli: a full transcriptomic profile | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2017-04-19T15:16:15Z | |
exeter.place-of-publication | England | en_GB |
dc.description | This is the author accepted manuscript. The final version is available from Taylor & Francis via the DOI in this record. | en_GB |
dc.identifier.journal | Nanotoxicology | en_GB |