A qualitative quantum rate model for hydrogen transfer in soybean lipoxygenase
Jevtic, S; Anders, J
Date: 1 September 2017
Journal
Journal of Chemical Physics
Publisher
AIP Publishing
Publisher DOI
Abstract
The hydrogen transfer reaction catalysed by soybean lipoxygenase (SLO) has been the focus of intense study following
observations of a high kinetic isotope effect (KIE). Today high KIEs are generally thought to indicate departure from
classical rate theory and are seen as a strong signature of tunnelling of the transferring particle, ...
The hydrogen transfer reaction catalysed by soybean lipoxygenase (SLO) has been the focus of intense study following
observations of a high kinetic isotope effect (KIE). Today high KIEs are generally thought to indicate departure from
classical rate theory and are seen as a strong signature of tunnelling of the transferring particle, hydrogen or one of its
isotopes, through the reaction energy barrier. In this paper we build a qualitative quantum rate model with few free
parameters that describes the dynamics of the transferring particle when it is exposed to energetic potentials exerted
by the donor and the acceptor. The enzyme’s impact on the dynamics is modelled by an additional energetic term, an
oscillatory contribution known as “gating”. By varying two key parameters, the gating frequency and the mean donoracceptor
separation, the model is able to reproduce well the KIE data for SLO wild-type and a variety of SLO mutants
over the experimentally accessible temperature range. While SLO-specific constants have been considered here, it is
possible to adapt these for other enzymes.
Physics and Astronomy
Faculty of Environment, Science and Economy
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