Monitoring of wild pseudomonas biofilm strain conditions using statistical characterization of scanning electron microscopy images
Dutta Sinha, S
Industrial and Engineering Chemistry Research
American Chemical Society (
Reason for embargo
Under embargo until 21/07/2018 in compliance with publisher policy
The present paper proposes a novel method of quantification of the variation in biofilm architecture, in correlation with the alteration of growth conditions that include variations of the substrate and conditioning layer. The polymeric biomaterials serving as substrates are widely used in implants and indwelling medical devices, while the plasma proteins serve as the conditioning layer. The present method uses descriptive statistics of field emission scanning electron microscopy (FESEM) images of biofilms obtained during a variety of growth conditions. We aim to explore here the texture and fractal analysis techniques, to identify the most discriminatory features which are capable of predicting the difference in biofilm growth conditions. We initially extract some statistical features of biofilm images on bare polymer surfaces, followed by those on the same substrates adsorbed with two different types of plasma proteins, viz., bovine serum albumin (BSA) and fibronectin (FN), for two different adsorption times. The present analysis has the potential to act as a futuristic technology for developing a computerized monitoring system in hospitals with automated image analysis and feature extraction, which may be used to predict the growth profile of an emerging biofilm on surgical implants or similar medical applications.
SDS acknowledges the funding from the Department of Science and Technology (DST), Govt. of India through the Women’s Scientist Scheme – A, project no. LS-466/WOS A/2012-2013.
This is the author accepted manuscript. The final version is available from the publisher via the DOI in this record.
Vol. 56, pp. 9496 - 9512