Mid-IR hyperspectral imaging for label-free histopathology and cytology
Hermes, M; Morrish, RB; Huot, L; et al.Meng, L; Junaid, S; Tomko, J; Lloyd, GR; Masselink, WT; Tidemand-Lichtenberg, P; Pedersen, C
Date: 24 January 2018
Article
Journal
Journal of Optics
Publisher
IOP Publishing
Publisher DOI
Abstract
Mid-infrared (MIR) imaging has emerged as a valuable tool to investigate biological samples,
such as tissue histological sections and cell cultures, by providing non-destructive chemical
specificity without recourse to labels. While feasibility studies have shown the capabilities of
MIR imaging approaches to address key biological ...
Mid-infrared (MIR) imaging has emerged as a valuable tool to investigate biological samples,
such as tissue histological sections and cell cultures, by providing non-destructive chemical
specificity without recourse to labels. While feasibility studies have shown the capabilities of
MIR imaging approaches to address key biological and clinical questions, these techniques are
still far from being deployable by non-expert users. In this review, we discuss the current state of
the art of MIR technologies and give an overview on technical innovations and developments
with the potential to make MIR imaging systems more readily available to a larger community.
The most promising developments over the last few years are discussed here. They include
improvements in MIR light sources with the availability of quantum cascade lasers and
supercontinuum IR sources as well as the recently developed upconversion scheme to improve
the detection of MIR radiation. These technical advances can substantially speed up data
acquisition of multispectral or hyperspectral datasets thus providing the end user with vast
amounts of data when imaging whole tissue areas of many mm2
. Therefore, effective data
analysis is of tremendous importance, and progress in method development is discussed with
respect to the specific biomedical context.
Physics and Astronomy
Faculty of Environment, Science and Economy
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