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dc.contributor.authorMansfield, Jessica
dc.contributor.authorMandalia, Vipul
dc.contributor.authorToms, Andrew
dc.contributor.authorWinlove, C. Peter
dc.contributor.authorBrasselet, Sophie
dc.date.accessioned2018-12-18T08:36:55Z
dc.date.issued2018-12-18
dc.description.abstractType II collagen fibre diameters in cartilage are beneath the diffraction limit of optical microscopy, which makes the assessment of collagen organization very challenging. In this work we use polarization sensitive second harmonic generation (P-SHG) imaging to map collagen organization in articular cartilage, addressing in particular its behaviour under strain and in osteoarthritic cartilage. P-SHG yields two parameters, molecular order and orientation, which provide measures of the degree of organisation both at the molecular scale (below the diffraction limit) and above a few hundreds of nanometers (at the image pixel size). P-SHG clearly demonstrates the zonal collagen architecture and reveals differences in the structure of the fibres around chondrocytes. P-SHG also reveals sub-micron scale fibre re-organisation in cartilage strips exposed to tensile loading, with an increase in local organization in the superficial zone which weakly correlates with tensile modulus. Finally, P-SHG is used to investigate osteoarthritic cartilage from total knee replacement surgery, and reveals widespread heterogeneity across samples both microscale fibre orientations and their sub-micron organisation. By addressing collagen fibre structure on scales intermediate between conventional light and electron microscopy, this study provides new insights into collagen micromechanics and mechanisms of degradation.en_GB
dc.description.sponsorshipArthritis Research UKen_GB
dc.description.sponsorshipLeverhulme Trust Visiting Professorshipen_GB
dc.identifier.doi10.24378/exe.603
dc.identifier.grantnumber20714en_GB
dc.identifier.grantnumberVP2-2015-025en_GB
dc.identifier.urihttp://hdl.handle.net/10871/35186
dc.language.isoenen_GB
dc.publisherUniversity of Exeteren_GB
dc.rightsCC BY 4.0en_GB
dc.subjectCartilage, Collagen, Second harmonic generation, Polarization, Micromechanicsen_GB
dc.titleCollagen reorganization in cartilage under strain probed by polarization sensitive Second Harmonic Generation microscopy (dataset)en_GB
dc.typeDataseten_GB
dc.date.available2018-12-18T08:36:55Z
dc.descriptionRaw image data, load cell data Matlab code for data analysisen_GB
dc.identifier.journalJournal of the Royal Society Interfaceen_GB
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_GB
dcterms.dateAccepted2018-12-18
rioxxterms.versionNAen_GB
rioxxterms.typeOtheren_GB
refterms.dateFOA2018-12-18T08:36:58Z


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