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Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader

dc.contributor.authorLiang, S
dc.contributor.authorKinghorn, AB
dc.contributor.authorVoliotis, M
dc.contributor.authorPrague, JK
dc.contributor.authorVeldhuis, JD
dc.contributor.authorTsaneva-Atanasova, K
dc.contributor.authorMcArdle, CA
dc.contributor.authorLi, RHW
dc.contributor.authorCass, AEG
dc.contributor.authorDhillo, WS
dc.contributor.authorTanner, JA
dc.date.accessioned2019-02-25T11:05:56Z
dc.date.issued2019-02-20
dc.description.abstractNormal reproductive functioning is critically dependent on pulsatile secretion of luteinising hormone (LH). Assessment of LH pulsatility is important for the clinical diagnosis of reproductive disorders, but current methods are hampered by frequent blood sampling coupled to expensive serial immunochemical analysis. Here, we report the development and application of a Robotic APTamer-enabled Electrochemical Reader (RAPTER) electrochemical analysis system to determine LH pulsatility. Through selective evolution of ligands by exponential enrichment (SELEX), we identify DNA aptamers that bind specifically to LH and not to related hormones. The aptamers are integrated into electrochemical aptamer-based (E-AB) sensors on a robotic platform. E-AB enables rapid, sensitive and repeatable determination of LH concentration profiles. Bayesian Spectrum Analysis is applied to determine LH pulsatility in three distinct patient cohorts. This technology has the potential to transform the clinical care of patients with reproductive disorders and could be developed to allow real-time in vivo hormone monitoring.en_GB
dc.description.sponsorshipHong Kong Health and Medical Research Fund (HMRF)en_GB
dc.description.sponsorshipNational Institute for Health Research (NIHR)en_GB
dc.description.sponsorshipUniversity of Hong Kong Outstanding Young Researcher Award 2015-16en_GB
dc.description.sponsorshipHKU Seed Fund for Translational and Applied Researchen_GB
dc.description.sponsorshipEngineering and Physical Sciences Research Council (EPSRC)en_GB
dc.description.sponsorshipMedical Research Council (MRC)en_GB
dc.identifier.citationVol. 10, 852en_GB
dc.identifier.doi10.1038/s41467-019-08799-6
dc.identifier.grantnumber03142546en_GB
dc.identifier.grantnumberRP-2014-05-001en_GB
dc.identifier.grantnumber201711160029en_GB
dc.identifier.grantnumberEP/ N014391/1.en_GB
dc.identifier.grantnumberMR/M024954/1en_GB
dc.identifier.urihttp://hdl.handle.net/10871/36046
dc.language.isoenen_GB
dc.publisherNature Researchen_GB
dc.relation.sourceThe authors declare that all data supporting the findings of this study are available within this published article, its Supplementary Information files and from the Source Data File. Supporting data are also available from the corresponding author upon reasonable request.en_GB
dc.relation.urlhttps://doi.org/10.1038/s41467-019-08799-6en_GB
dc.rightsOpen Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/. © The Author(s) 2019en_GB
dc.titleMeasuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical readeren_GB
dc.typeArticleen_GB
dc.date.available2019-02-25T11:05:56Z
dc.identifier.issn2041-1723
dc.descriptionThis is the final version. Available from the publisher via the DOI in this record.en_GB
dc.identifier.journalNature Communicationsen_GB
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/.en_GB
dcterms.dateAccepted2019-01-28
rioxxterms.versionVoRen_GB
rioxxterms.licenseref.startdate2019-02-20
rioxxterms.typeJournal Article/Reviewen_GB
refterms.dateFCD2019-02-25T11:00:51Z
refterms.versionFCDVoR
refterms.dateFOA2019-02-25T11:05:59Z
refterms.panelBen_GB


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Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/. © The Author(s) 2019
Except where otherwise noted, this item's licence is described as Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/. © The Author(s) 2019