Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader
dc.contributor.author | Liang, S | |
dc.contributor.author | Kinghorn, AB | |
dc.contributor.author | Voliotis, M | |
dc.contributor.author | Prague, JK | |
dc.contributor.author | Veldhuis, JD | |
dc.contributor.author | Tsaneva-Atanasova, K | |
dc.contributor.author | McArdle, CA | |
dc.contributor.author | Li, RHW | |
dc.contributor.author | Cass, AEG | |
dc.contributor.author | Dhillo, WS | |
dc.contributor.author | Tanner, JA | |
dc.date.accessioned | 2019-02-25T11:05:56Z | |
dc.date.issued | 2019-02-20 | |
dc.description.abstract | Normal reproductive functioning is critically dependent on pulsatile secretion of luteinising hormone (LH). Assessment of LH pulsatility is important for the clinical diagnosis of reproductive disorders, but current methods are hampered by frequent blood sampling coupled to expensive serial immunochemical analysis. Here, we report the development and application of a Robotic APTamer-enabled Electrochemical Reader (RAPTER) electrochemical analysis system to determine LH pulsatility. Through selective evolution of ligands by exponential enrichment (SELEX), we identify DNA aptamers that bind specifically to LH and not to related hormones. The aptamers are integrated into electrochemical aptamer-based (E-AB) sensors on a robotic platform. E-AB enables rapid, sensitive and repeatable determination of LH concentration profiles. Bayesian Spectrum Analysis is applied to determine LH pulsatility in three distinct patient cohorts. This technology has the potential to transform the clinical care of patients with reproductive disorders and could be developed to allow real-time in vivo hormone monitoring. | en_GB |
dc.description.sponsorship | Hong Kong Health and Medical Research Fund (HMRF) | en_GB |
dc.description.sponsorship | National Institute for Health Research (NIHR) | en_GB |
dc.description.sponsorship | University of Hong Kong Outstanding Young Researcher Award 2015-16 | en_GB |
dc.description.sponsorship | HKU Seed Fund for Translational and Applied Research | en_GB |
dc.description.sponsorship | Engineering and Physical Sciences Research Council (EPSRC) | en_GB |
dc.description.sponsorship | Medical Research Council (MRC) | en_GB |
dc.identifier.citation | Vol. 10, 852 | en_GB |
dc.identifier.doi | 10.1038/s41467-019-08799-6 | |
dc.identifier.grantnumber | 03142546 | en_GB |
dc.identifier.grantnumber | RP-2014-05-001 | en_GB |
dc.identifier.grantnumber | 201711160029 | en_GB |
dc.identifier.grantnumber | EP/ N014391/1. | en_GB |
dc.identifier.grantnumber | MR/M024954/1 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/36046 | |
dc.language.iso | en | en_GB |
dc.publisher | Nature Research | en_GB |
dc.relation.source | The authors declare that all data supporting the findings of this study are available within this published article, its Supplementary Information files and from the Source Data File. Supporting data are also available from the corresponding author upon reasonable request. | en_GB |
dc.relation.url | https://doi.org/10.1038/s41467-019-08799-6 | en_GB |
dc.rights | Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/. © The Author(s) 2019 | en_GB |
dc.title | Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2019-02-25T11:05:56Z | |
dc.identifier.issn | 2041-1723 | |
dc.description | This is the final version. Available from the publisher via the DOI in this record. | en_GB |
dc.identifier.journal | Nature Communications | en_GB |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_GB |
dcterms.dateAccepted | 2019-01-28 | |
rioxxterms.version | VoR | en_GB |
rioxxterms.licenseref.startdate | 2019-02-20 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2019-02-25T11:00:51Z | |
refterms.versionFCD | VoR | |
refterms.dateFOA | 2019-02-25T11:05:59Z | |
refterms.panel | B | en_GB |
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Commons license, and indicate if changes were made. The images or other third party
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indicated otherwise in a credit line to the material. If material is not included in the
article’s Creative Commons license and your intended use is not permitted by statutory
regulation or exceeds the permitted use, you will need to obtain permission directly from
the copyright holder. To view a copy of this license, visit http://creativecommons.org/
licenses/by/4.0/.
© The Author(s) 2019