Comparing the selective and co-selective effects of different antimicrobials in bacterial communities
dc.contributor.author | Murray, AK | |
dc.contributor.author | Zhang, L | |
dc.contributor.author | Snape, J | |
dc.contributor.author | Gaze, W | |
dc.date.accessioned | 2019-03-14T10:17:32Z | |
dc.date.issued | 2019-03-16 | |
dc.description.abstract | Bacterial communities are exposed to a cocktail of antimicrobial agents, including antibiotics, heavy metals and biocidal antimicrobials such as quaternary ammonium compounds (QACs). The extent to which these compounds may select or co-select for antimicrobial resistance (AMR) is not fully understood. In this study, human associated, wastewater derived, bacterial communities were exposed to either benzalkonium chloride (BAC), ciprofloxacin or trimethoprim at sub-point of use concentrations for one week, in order to determine selective and co-selective potential. Metagenome analyses were performed to determine effects on bacterial community structure and prevalence of antibiotic resistance genes (ARGs) and metal or biocide resistance genes (MBRGS). Ciprofloxacin had the greatest co-selective potential, significantly enriching for resistance mechanisms to multiple antibiotic classes. Conversely, BAC exposure significantly reduced relative abundance of ARGs and MBRGS, including the well characterised qac efflux genes. However, BAC exposure significantly impacted bacterial community structure. This suggests BAC and potentially other QACs did not play as significant a role in co-selection for AMR relative to antibiotics such as ciprofloxacin at below point of use concentrations in this study. This approach can be used to identify priority compounds for further study, to better understand evolution of AMR in bacterial communities exposed to sub-point of use concentrations of antimicrobials. | en_GB |
dc.description.sponsorship | Natural Environment Research Council (NERC) | en_GB |
dc.description.sponsorship | Biotechnology and Biological Sciences Research Council (BBSRC) | en_GB |
dc.identifier.citation | Published online 16 March 2019 | en_GB |
dc.identifier.doi | 10.1016/j.ijantimicag.2019.03.001 | |
dc.identifier.grantnumber | NE/R01373X/1 | en_GB |
dc.identifier.grantnumber | BB/L502509/1 | en_GB |
dc.identifier.uri | http://hdl.handle.net/10871/36462 | |
dc.language.iso | en | en_GB |
dc.publisher | Elsevier | en_GB |
dc.rights | © 2019. This version is made available under the CC-BY-NC-ND 4.0 license: https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | antibiotic | en_GB |
dc.subject | antimicrobial | en_GB |
dc.subject | biocide | en_GB |
dc.subject | quaternary ammonium compound | en_GB |
dc.subject | resistance | en_GB |
dc.subject | evolution | en_GB |
dc.subject | metagenomics | en_GB |
dc.title | Comparing the selective and co-selective effects of different antimicrobials in bacterial communities | en_GB |
dc.type | Article | en_GB |
dc.date.available | 2019-03-14T10:17:32Z | |
dc.identifier.issn | 0924-8579 | |
dc.description | This is the author accepted manuscript. The final version is available on open access from Elsevier via the DOI in this record | en_GB |
dc.identifier.journal | International Journal of Antimicrobial Agents | en_GB |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | en_GB |
dcterms.dateAccepted | 2019-03-04 | |
exeter.funder | ::Natural Environment Research Council (NERC) | en_GB |
rioxxterms.version | AM | en_GB |
rioxxterms.licenseref.startdate | 2019-03-04 | |
rioxxterms.type | Journal Article/Review | en_GB |
refterms.dateFCD | 2019-03-13T16:05:16Z | |
refterms.versionFCD | AM | |
refterms.dateFOA | 2019-03-22T15:29:51Z | |
refterms.panel | A | en_GB |
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Except where otherwise noted, this item's licence is described as © 2019. This version is made available under the CC-BY-NC-ND 4.0 license: https://creativecommons.org/licenses/by-nc-nd/4.0/