Compounds that select against the tetracycline-resistance efflux pump
Stone, LK; Baym, M; Lieberman, TD; et al.Chait, R; Clardy, J; Kishony, R
Date: 19 September 2016
Journal
Nature Chemical Biology
Publisher
Nature Research
Publisher DOI
Abstract
We developed a competition-based screening strategy to identify compounds that invert the selective advantage of antibiotic resistance. Using our assay, we screened over 19,000 compounds for the ability to select against the TetA tetracycline-resistance efflux pump in Escherichia coli and identified two hits, β-thujaplicin and disulfiram. ...
We developed a competition-based screening strategy to identify compounds that invert the selective advantage of antibiotic resistance. Using our assay, we screened over 19,000 compounds for the ability to select against the TetA tetracycline-resistance efflux pump in Escherichia coli and identified two hits, β-thujaplicin and disulfiram. Treating a tetracycline-resistant population with β-thujaplicin selects for loss of the resistance gene, enabling an effective second-phase treatment with doxycycline.
Biosciences - old structure
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