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Compounds that select against the tetracycline-resistance efflux pump

dc.contributor.authorStone, LK
dc.contributor.authorBaym, M
dc.contributor.authorLieberman, TD
dc.contributor.authorChait, R
dc.contributor.authorClardy, J
dc.contributor.authorKishony, R
dc.date.accessioned2020-01-09T16:03:46Z
dc.date.issued2016-09-19
dc.description.abstractWe developed a competition-based screening strategy to identify compounds that invert the selective advantage of antibiotic resistance. Using our assay, we screened over 19,000 compounds for the ability to select against the TetA tetracycline-resistance efflux pump in Escherichia coli and identified two hits, β-thujaplicin and disulfiram. Treating a tetracycline-resistant population with β-thujaplicin selects for loss of the resistance gene, enabling an effective second-phase treatment with doxycycline.en_GB
dc.description.sponsorshipNational Institute of Allergy and Infectious Diseasesen_GB
dc.description.sponsorshipUS National Institutes of Healthen_GB
dc.description.sponsorshipEuropean Union FP7en_GB
dc.description.sponsorshipNational Science Foundationen_GB
dc.identifier.citationVol. 12, pp. 902 - 904en_GB
dc.identifier.doi10.1038/nchembio.2176
dc.identifier.grantnumberU54 AI057159en_GB
dc.identifier.grantnumberR01 GM081617en_GB
dc.identifier.grantnumberGM086258en_GB
dc.identifier.grantnumber281891en_GB
dc.identifier.urihttp://hdl.handle.net/10871/40340
dc.language.isoenen_GB
dc.publisherNature Researchen_GB
dc.rights© 2016 Nature America, Inc. All rights reserveden_GB
dc.titleCompounds that select against the tetracycline-resistance efflux pumpen_GB
dc.typeArticleen_GB
dc.date.available2020-01-09T16:03:46Z
dc.identifier.issn1552-4450
dc.descriptionThis is the author accepted manuscript. The final version is available from Nature Research via the DOI in this recorden_GB
dc.descriptionAccession codes. The sequences reported in this article have been deposited in the National Center for Biotechnology Information Sequence Read Archive database (accession number SRP073071).en_GB
dc.identifier.journalNature Chemical Biologyen_GB
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserveden_GB
dcterms.dateAccepted2016-07-14
rioxxterms.versionAMen_GB
rioxxterms.licenseref.startdate2016-09-19
rioxxterms.typeJournal Article/Reviewen_GB
refterms.dateFCD2020-01-09T16:00:33Z
refterms.versionFCDAM
refterms.dateFOA2020-01-09T16:03:55Z
refterms.panelAen_GB


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