Chemical and Biochemical Silica Surface Modifications for the Development of Medical Analyses

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Chemical and Biochemical Silica Surface Modifications for the Development of Medical Analyses

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dc.contributor.author Kretzers, Ivo en_GB
dc.date.accessioned 2009-09-29T11:53:24Z en_GB
dc.date.accessioned 2011-01-26T09:43:28Z en_US
dc.date.accessioned 2013-03-20T18:43:08Z
dc.date.issued 2009-03-31 en_GB
dc.description.abstract This report can be divided into two parts. The first part studying the aggregation kinetics for citrate-reduced 15 nm gold nanoparticles at the native silica and modified silica-water interfaces. At the native, negatively charged silica-water interfaces a two-phase adsorption is observed: a pseudo-Langmurian adsorption phase and, after an acid wash to remove the citrate ligand from the adsorbed particles, a further pseudo-Langmurian adsorption phase. A kinetic analysis of these phases shows an average adsorption rate constant of (2.0 ± 2.5)  105 M 1 s 1 with no measurable desorption. The second part of this report describes protocol development for tethering DNA onto gold nanoparticles and the development of hybridization procedures. 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-Hydroxysuccinimide (EDC/NHS) activation of a thioctic acid monolayer on a gold nanoparticle shows the most promising method. en_GB
dc.identifier.uri http://hdl.handle.net/10036/82938 en_GB
dc.language.iso en en_GB
dc.publisher University of Exeter en_GB
dc.title Chemical and Biochemical Silica Surface Modifications for the Development of Medical Analyses en_GB
dc.type Thesis or dissertation en_GB
dc.date.available 2009-09-29T11:53:24Z en_GB
dc.date.available 2011-01-26T09:43:28Z en_US
dc.date.available 2013-03-20T18:43:08Z
dc.contributor.advisor Shaw, Andrew en_GB
dc.publisher.department Biosciences en_GB
dc.type.degreetitle MPhil in Biological Sciences en_GB
dc.type.qualificationlevel Masters Degree en_GB
dc.type.qualificationname MPhil en_GB


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