The use of zebrafish embryos as an alternative approach for ecotoxicity testing

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The use of zebrafish embryos as an alternative approach for ecotoxicity testing

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dc.contributor.author Lillicrap, Adam en_GB
dc.date.accessioned 2010-03-15T10:41:34Z en_GB
dc.date.accessioned 2011-01-26T09:43:21Z en_US
dc.date.accessioned 2013-03-20T18:43:04Z
dc.date.issued 2009-03-31 en_GB
dc.description.abstract The use of fish embryos for acute ecotoxicity tests has been widely documented and over recent years there has been significant progress in the use of zebrafish embryos as an alternative approach to juvenile fish. However, there are still some questions preventing the unilateral adoption of this surrogate life stage as an alternative to the use of juvenile fish for regulatory testing purposes. Many of the concerns that have been raised include the absolute sensitivity of fish embryos. For example, published work has shown that fish embryos are not as sensitive as other more developed life-stages (such as the eleutheroembryos) to certain classes of compounds including, but not exclusively, cationic polymers. However, there is limited understanding of why fish embryos do not behave similarly and exhibit a toxic response to these classes of chemicals. One possible reason is that fish embryos have a protective envelope called a chorion, which could prevent these certain chemicals from passing into the embryo. The research described within this thesis has focussed on understanding the need for alternative approaches in ecotoxicity testing, particularly regarding the use of fish embryos. In addition, the developmental ontogeny of zebrafish embryos has also been studied. Finally, the research investigated whether the chorion is indeed a barrier to entry to certain chemicals, and a technique for quantifying the proportion of bio-available chemical within the different components of the embryo was developed. en_GB
dc.description.sponsorship Astrazeneca en_GB
dc.identifier.uri http://hdl.handle.net/10036/94256 en_GB
dc.language.iso en en_GB
dc.publisher University of Exeter en_GB
dc.subject zebrafish en_GB
dc.subject embryo en_GB
dc.subject uptake en_GB
dc.subject ecotoxicology en_GB
dc.title The use of zebrafish embryos as an alternative approach for ecotoxicity testing en_GB
dc.type Thesis or dissertation en_GB
dc.date.available 2010-03-15T10:41:34Z en_GB
dc.date.available 2011-01-26T09:43:21Z en_US
dc.date.available 2013-03-20T18:43:04Z
dc.contributor.advisor Tyler, Charles en_GB
dc.contributor.advisor Williams, Tim en_GB
dc.publisher.department Biosciences en_GB
dc.type.degreetitle Master of Philosophy in Biological Sciences en_GB
dc.type.qualificationlevel Masters Degree en_GB
dc.type.qualificationname MPhil en_GB


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